@article{c1bb9fc3c08f4e2595c1ea13708402a1,
title = "The mitochondrial elongation factor LeEF-Tsmt is regulated during tomato fruit ripening and upon wounding and ethylene treatment",
abstract = "A gene encoding an elongation factor LeEF-Tsmt that participates in the protein synthesis process in mitochondria shows strong expression in ripening fruit as compared to other organs. It is strongly up-regulated during the first stages of the ripening process in parallel with the climacteric rise in respiration. LeEF-Tsmt expression is stimulated by ethylene, wounding and high temperature but ethylene-insensitive mutants exhibit normal expression. Transgenic fruit have been generated in which LeEF-Tsmt has been constitutively up- and down-regulated. Surprisingly, altering the expression of the gene by genetic transformation with antisense and sense LeEF-Tsmt constructs did not affect the pattern of respiration and ethylene production during ripening and upon wounding. In addition, expression of the alternative oxidase gene which is known to play an important role in respiratory climacteric was not affected. Possible reasons for the absence of effect on respiration of variations of LeEF-Tsmt gene expression are discussed.",
keywords = "Ethylene, Lycopersicon esculentum, Mitochondrial elongation factor, Respiration, Transgenic plants, Wounding",
author = "Girardi, {Cesar Luis} and Kalina Bermudez and Anne Bernadac and Ana Chavez and Mohamed Zouine and Miranda, {Fabiola Jaimes} and Mondher Bouzayen and Pech, {Jean Claude} and Alain Latch{\'e}",
note = "Funding Information: The authors wish to thank P. Maury and O. Berseille for their participation in the determination of respiratory activity and RNA extractions respectively. H. Mondies and L. Tessarotto provided excellent technical assistance for the generation the transgenic plants. Agrofresh provided 1-MCP. This research was partly supported by the Midi-Pyr{\'e}n{\'e}es Regional Council (grants no 01008920 and 03001146). C.L. Girardi and A. Chavez received a bursary from the government of Brazil (CAPES). F. Jaimes-Miranda and K. Bermudez received a doctoral and postdoctoral fellowship from CONACYT and Instituto Politecnico Nacional and COFAA, respectively. C.L. Girardi is from EMBRAPA UVA E VINHO (Bento Gon{\c c}alves, RS, Brazil) and this work represents some of the research submitted in partial fulfilment of the requirements for its doctorate degree.",
year = "2006",
month = oct,
doi = "10.1016/j.postharvbio.2006.06.002",
language = "Ingl{\'e}s",
volume = "42",
pages = "1--7",
journal = "Postharvest Biology and Technology",
issn = "0925-5214",
number = "1",
}