TY - JOUR
T1 - The KISS1 gene overexpression as a potential molecular marker for cervical cancer cells
AU - Taniguchi-Ponciano, Keiko
AU - Ribas-Aparicio, Rosa María
AU - Marrero-Rodríguez, Daniel
AU - Arreola-De La Cruz, Hugo
AU - Huerta-Padilla, Víctor
AU - Muñoz, Nancy
AU - Gómez-Ortiz, Laura
AU - Ponce-Navarrete, Gustavo
AU - Rodríguez-Esquivel, Miriam
AU - Mendoza-Rodríguez, Mónica
AU - Gómez-Virgilio, Laura
AU - Peralta, Raúl
AU - Serna, Luis
AU - Gómez, Guillermo
AU - Ortiz, Jorge
AU - Mantilla, Alejandra
AU - Hernández, Daniel
AU - Hernández, Ángeles
AU - Bandala, Cindy
AU - Salcedo, Mauricio
N1 - Publisher Copyright:
© 2018 - IOS Press and the authors. All rights reserved.
PY - 2018/8/20
Y1 - 2018/8/20
N2 - BACKGROUND: Similarities between the pathologic progression of cancer and the physiologic process of placentation have been recognized for many years proposing that both present similar mechanisms and processes. Cervical cancer (CC) is one of the most frequent neoplasia among Mexican women turning it into an important health problem. OBJECTIVE: The aim of this study was to determine the degree of the involvement of pregnancy related genes and in cancer progression by in-silico analysis and validated in CC samples. RESULTS: The data mining analysis resulted in the identification of genes expressed in term placenta, first trimester placenta and normal cervical tissues. Finally, we selected KISS1 for the involvement of pregnancy related gene and also in cancer process. In order to explore KISS1 in CC, we analyzed Copy Number Variation (CNV) and gene expression using microarray experiments. KISS1 showed 20% genomic gain in 1q32.1 on CC samples. Furthermore, microarray analysis showed KISS1 as up-regulated genes. Results were validated showing an overexpression of 85% of KISS1 in CC samples. CONCLUSIONS: Data suggest KISS1 as a great candidate for CC molecular markers or as a therapeutic target for CC. Also, HPV presence does not seem to alter the KISS1 expression in CC.
AB - BACKGROUND: Similarities between the pathologic progression of cancer and the physiologic process of placentation have been recognized for many years proposing that both present similar mechanisms and processes. Cervical cancer (CC) is one of the most frequent neoplasia among Mexican women turning it into an important health problem. OBJECTIVE: The aim of this study was to determine the degree of the involvement of pregnancy related genes and in cancer progression by in-silico analysis and validated in CC samples. RESULTS: The data mining analysis resulted in the identification of genes expressed in term placenta, first trimester placenta and normal cervical tissues. Finally, we selected KISS1 for the involvement of pregnancy related gene and also in cancer process. In order to explore KISS1 in CC, we analyzed Copy Number Variation (CNV) and gene expression using microarray experiments. KISS1 showed 20% genomic gain in 1q32.1 on CC samples. Furthermore, microarray analysis showed KISS1 as up-regulated genes. Results were validated showing an overexpression of 85% of KISS1 in CC samples. CONCLUSIONS: Data suggest KISS1 as a great candidate for CC molecular markers or as a therapeutic target for CC. Also, HPV presence does not seem to alter the KISS1 expression in CC.
KW - KISS1
KW - cervical cancer
KW - in-silico analysis
KW - molecular marker
UR - http://www.scopus.com/inward/record.url?scp=85056267742&partnerID=8YFLogxK
U2 - 10.3233/CBM-181215
DO - 10.3233/CBM-181215
M3 - Artículo
C2 - 29914007
SN - 1574-0153
VL - 22
SP - 709
EP - 719
JO - Cancer Biomarkers
JF - Cancer Biomarkers
IS - 4
ER -