TY - JOUR
T1 - The detection of inherent homologous recombination between repeat sequences in H. pylori 26695 by the PCR-based method
AU - Fu, Yajuan
AU - Zepeda-Gurrola, Reyna Cristina
AU - Aguilar-Gutiérrez, Germán Rubén
AU - Lara-Ramírez, Edgar E.
AU - De Luna-Santillana, Erick J.
AU - Rodríguez-Luna, Isabel Cristina
AU - Sánchez-Varela, Alejandro
AU - Carreño-López, Ricardo
AU - Moreno-Medina, Víctor Ricardo
AU - Rodríguez-Pérez, Mario A.
AU - López-Vidal, Yolanda
AU - Guo, Xianwu
N1 - Funding Information:
The authors are very grateful to Dr. Nina R. Salama for her kind help in providing them some genomic DNA of Helicobacter pylori. Xianwu Guo and Mario A. Rodríguez-Pérez hold a scholarship from Comisión de Operación y Fomento de Actividades Académicas/Instituto Politécnico Nacional. This work was supported by the Consejo Nacional de Ciencia y Tecnología-México ( http://www.conacyt.gob.mx/convocatorias/Paginas/default.aspx ) (Fondo Sectorial de Investigación Básica SEP CONACyT (CB-2011-01) with Grant number: 168541); and Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional, México ( http://www.sip.ipn.mx/WPS/WCM/CONNECT/SIP/SIP/INICIO/INDEX.HTM ) (Grant numbers: SIP20120202).
PY - 2014/2
Y1 - 2014/2
N2 - Helicobacter pylori infects more than half of the world's population, making it the most widespread infection of bacteria. It has high genetic diversity and has been considered as one of the most variable bacterial species. In the present study, a PCR-based method was used to detect the presence and the relative frequency of homologous recombination between repeat sequences (<500 bp) in H. pylori 26695. All the recombinant structures have been confirmed by sequencing. The inversion generated between inverted repeats showed distinct features from the recombination for duplication or deletion between direct repeats. Meanwhile, we gave the mathematic reasoning of a general formula for the calculation of relative recombination frequency and indicated the conditions for its application. This formula could be extensively applied to detect the frequency of homologous recombination, site-specific recombination, and other types of predictable recombination. Our results should be helpful for better understanding the genome evolution and adaptation of bacteria.
AB - Helicobacter pylori infects more than half of the world's population, making it the most widespread infection of bacteria. It has high genetic diversity and has been considered as one of the most variable bacterial species. In the present study, a PCR-based method was used to detect the presence and the relative frequency of homologous recombination between repeat sequences (<500 bp) in H. pylori 26695. All the recombinant structures have been confirmed by sequencing. The inversion generated between inverted repeats showed distinct features from the recombination for duplication or deletion between direct repeats. Meanwhile, we gave the mathematic reasoning of a general formula for the calculation of relative recombination frequency and indicated the conditions for its application. This formula could be extensively applied to detect the frequency of homologous recombination, site-specific recombination, and other types of predictable recombination. Our results should be helpful for better understanding the genome evolution and adaptation of bacteria.
UR - http://www.scopus.com/inward/record.url?scp=84898854996&partnerID=8YFLogxK
U2 - 10.1007/s00284-013-0466-7
DO - 10.1007/s00284-013-0466-7
M3 - Artículo
SN - 0343-8651
VL - 68
SP - 211
EP - 219
JO - Current Microbiology
JF - Current Microbiology
IS - 2
ER -