TY - JOUR
T1 - The Aggregation Pheromone of Metamasius spinolae (Coleoptera: Dryophthoridae) Revisited
T2 - Less is More
AU - Malo, Edi A.
AU - Gutiérrez-Escobar, Verónica
AU - Castrejón-Ayala, Federico
AU - Rojas, Julio C.
AU - Stelinski, Lukasz
N1 - Publisher Copyright:
© 2020 The Author(s). Published by Oxford University Press on behalf of Entomological Society of America. All rights reserved.
PY - 2020/8/1
Y1 - 2020/8/1
N2 - The weevil Metamasius spinolae (Gyllenhal) is the most important insect pest of cultivated prickly pear in Mexico. A previous work reported that the pheromone of this weevil species was composed by three components. In this study, we reinvestigated the aggregation pheromone of M. spinolae using gas chromatography-electroantennography and gas chromatography-mass spectrometry to locate and identify new potential pheromonal compounds. The behavioral activity of identified compounds was evaluated in laboratory and field trials. Metamasius spinolae males released four compounds: 2-methyl-4-heptanone, 6-methyl-2-hepten-4-one, 2-methyl-4-octanone, and 2-hydroxy-2-methyl-4-heptanone. In the laboratory assays, depending on the concentration, the compounds were attractive, neutral, or repellent to M. spinolae. Field evaluation showed that traps baited with 2-hydroxy-2-methyl-4-heptanone singly or in most of the binary or tertiary blends where this compound was present captured a higher number of M. spinolae compared to live males and the other compounds identified. In conclusion, our results indicate that 2-hydroxy-2-methyl-4-heptanone is the main component of the aggregation pheromone of M. spinolae. We suggest that this compound should be used for developing a monitoring or a mass-trapping system for M. spinolae.
AB - The weevil Metamasius spinolae (Gyllenhal) is the most important insect pest of cultivated prickly pear in Mexico. A previous work reported that the pheromone of this weevil species was composed by three components. In this study, we reinvestigated the aggregation pheromone of M. spinolae using gas chromatography-electroantennography and gas chromatography-mass spectrometry to locate and identify new potential pheromonal compounds. The behavioral activity of identified compounds was evaluated in laboratory and field trials. Metamasius spinolae males released four compounds: 2-methyl-4-heptanone, 6-methyl-2-hepten-4-one, 2-methyl-4-octanone, and 2-hydroxy-2-methyl-4-heptanone. In the laboratory assays, depending on the concentration, the compounds were attractive, neutral, or repellent to M. spinolae. Field evaluation showed that traps baited with 2-hydroxy-2-methyl-4-heptanone singly or in most of the binary or tertiary blends where this compound was present captured a higher number of M. spinolae compared to live males and the other compounds identified. In conclusion, our results indicate that 2-hydroxy-2-methyl-4-heptanone is the main component of the aggregation pheromone of M. spinolae. We suggest that this compound should be used for developing a monitoring or a mass-trapping system for M. spinolae.
KW - cactus
KW - chemical communication
KW - male volatile
KW - prickly pear
KW - weevil
UR - http://www.scopus.com/inward/record.url?scp=85089787454&partnerID=8YFLogxK
U2 - 10.1093/ee/nvaa054
DO - 10.1093/ee/nvaa054
M3 - Artículo
C2 - 32457987
AN - SCOPUS:85089787454
SN - 0046-225X
VL - 49
SP - 803
EP - 809
JO - Environmental Entomology
JF - Environmental Entomology
IS - 4
ER -