Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions

Rogelio Jimenez Juarez; Gabriela  Martínez Mejía; Nadia Adriana Vázquez-Torres; Andrés Castell-Rodríguez; José Manuel del Río; Mónica Corea

doi:doi.org/10.1016/j.colsurfa.2019.123658

Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions

Título traducido de la contribución: Síntesis de nuevos andamios de quitosano-glutaraldehído para ingeniería de tejidos usando reacciones de Schiff

Rogelio Jimenez Juarez, Gabriela Martínez Mejía, Nadia Adriana Vázquez-Torres, Andrés Castell-Rodríguez, José Manuel del Río, Mónica Corea

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

Resumen

Hydrogels of chitosan (CS) and glutaraldehyde (GA) were synthesized for tissue engineering applications by using a Schiff reaction. CS was reacted with GA (a cross-linker) at different concentrations, which were expressed as percentage of weight (2, 4, 6, 8 and 10 wt%). An evaluation was made of the effect of the different wt% of GA on the swelling and rheological properties of the hydrogels. The Schiff crosslinking reaction was monitored by UV–vis spectroscopy (550 nm) to determine the reaction kinetics and reaction order at 60 °C. The hydrogel structures were characterized by NMR, FT-IR, HR-MS and SEM, while the degree of cross-linking was examined with TGA-DA. The smaller pores and greatest swelling were found in hydrogels containing 10 wt% of GA. However, only the hydrogels with GA at 2, 4 and 6 wt% displayed viable cells, indicating their in vitro cyto- compatibility. The rheological studies showed that the values of the loss and storage modules in the hydrogels

Título traducido de la contribución	Síntesis de nuevos andamios de quitosano-glutaraldehído para ingeniería de tejidos usando reacciones de Schiff
Idioma original	Inglés
Número de artículo	123658
Páginas (desde-hasta)	1-15
Número de páginas	15
Publicación	Colloids and Surfaces A: Physicochemical and Engineering Aspects
Volumen	579
N.º	123658
DOI	https://doi.org/doi.org/10.1016/j.colsurfa.2019.123658
Estado	Publicada - 13 jul. 2019

Palabras clave

CS, chitosan; GA, glutaraldehyde; DNP, 2,4-dinitrophenylhydrazine; wt%, percentage of weight; UV-vis, ultraviolet-visible spectroscopy; FT-IR, Fourier-transform infrared spectroscopy; SEM, scanning electron microcopy; DA-TGA, derivate thermogravimetric analysis

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doi.org/10.1016/j.colsurfa.2019.123658

Citar esto

Jimenez Juarez, R., Martínez Mejía, G., Vázquez-Torres, N. A., Castell-Rodríguez, A., del Río, J. M., & Corea, M. (2019). Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions. Colloids and Surfaces A: Physicochemical and Engineering Aspects, 579(123658), 1-15. Artículo 123658. https://doi.org/doi.org/10.1016/j.colsurfa.2019.123658

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title = "Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions",

abstract = "Hydrogels of chitosan (CS) and glutaraldehyde (GA) were synthesized for tissue engineering applications by using a Schiff reaction. CS was reacted with GA (a cross-linker) at different concentrations, which were expressed as percentage of weight (2, 4, 6, 8 and 10 wt%). An evaluation was made of the effect of the different wt% of GA on the swelling and rheological properties of the hydrogels. The Schiff crosslinking reaction was monitored by UV–vis spectroscopy (550 nm) to determine the reaction kinetics and reaction order at 60 °C. The hydrogel structures were characterized by NMR, FT-IR, HR-MS and SEM, while the degree of cross-linking was examined with TGA-DA. The smaller pores and greatest swelling were found in hydrogels containing 10 wt% of GA. However, only the hydrogels with GA at 2, 4 and 6 wt% displayed viable cells, indicating their in vitro cyto- compatibility. The rheological studies showed that the values of the loss and storage modules in the hydrogels",

keywords = "CS, chitosan; GA, glutaraldehyde; DNP, 2,4-dinitrophenylhydrazine; wt%, percentage of weight; UV-vis, ultraviolet-visible spectroscopy; FT-IR, Fourier-transform infrared spectroscopy; SEM, scanning electron microcopy; DA-TGA, derivate thermogravimetric analysis",

author = "{Jimenez Juarez}, Rogelio and {Mart{\'i}nez Mej{\'i}a}, Gabriela and V{\'a}zquez-Torres, {Nadia Adriana} and Andr{\'e}s Castell-Rodr{\'i}guez and {del R{\'i}o}, {Jos{\'e} Manuel} and M{\'o}nica Corea",

year = "2019",

month = jul,

day = "13",

doi = "doi.org/10.1016/j.colsurfa.2019.123658",

language = "Ingl{\'e}s",

volume = "579",

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journal = "Colloids and Surfaces A: Physicochemical and Engineering Aspects",

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Jimenez Juarez, R , Martínez Mejía, G, Vázquez-Torres, NA, Castell-Rodríguez, A, del Río, JM & Corea, M 2019, 'Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions', Colloids and Surfaces A: Physicochemical and Engineering Aspects, vol. 579, n.º 123658, 123658, pp. 1-15. https://doi.org/doi.org/10.1016/j.colsurfa.2019.123658

Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions. / Jimenez Juarez, Rogelio ; Martínez Mejía, Gabriela; Vázquez-Torres, Nadia Adriana et al.
En: Colloids and Surfaces A: Physicochemical and Engineering Aspects, Vol. 579, N.º 123658, 123658, 13.07.2019, p. 1-15.

Producción científica: Contribución a una revista › Artículo › revisión exhaustiva

TY - JOUR

T1 - Synthesis of new chitosan-glutaraldehyde scaffolds for tissue engineering using Schiff reactions

AU - Jimenez Juarez, Rogelio

AU - Martínez Mejía, Gabriela

AU - Vázquez-Torres, Nadia Adriana

AU - Castell-Rodríguez, Andrés

AU - del Río, José Manuel

AU - Corea, Mónica

PY - 2019/7/13

Y1 - 2019/7/13

N2 - Hydrogels of chitosan (CS) and glutaraldehyde (GA) were synthesized for tissue engineering applications by using a Schiff reaction. CS was reacted with GA (a cross-linker) at different concentrations, which were expressed as percentage of weight (2, 4, 6, 8 and 10 wt%). An evaluation was made of the effect of the different wt% of GA on the swelling and rheological properties of the hydrogels. The Schiff crosslinking reaction was monitored by UV–vis spectroscopy (550 nm) to determine the reaction kinetics and reaction order at 60 °C. The hydrogel structures were characterized by NMR, FT-IR, HR-MS and SEM, while the degree of cross-linking was examined with TGA-DA. The smaller pores and greatest swelling were found in hydrogels containing 10 wt% of GA. However, only the hydrogels with GA at 2, 4 and 6 wt% displayed viable cells, indicating their in vitro cyto- compatibility. The rheological studies showed that the values of the loss and storage modules in the hydrogels

AB - Hydrogels of chitosan (CS) and glutaraldehyde (GA) were synthesized for tissue engineering applications by using a Schiff reaction. CS was reacted with GA (a cross-linker) at different concentrations, which were expressed as percentage of weight (2, 4, 6, 8 and 10 wt%). An evaluation was made of the effect of the different wt% of GA on the swelling and rheological properties of the hydrogels. The Schiff crosslinking reaction was monitored by UV–vis spectroscopy (550 nm) to determine the reaction kinetics and reaction order at 60 °C. The hydrogel structures were characterized by NMR, FT-IR, HR-MS and SEM, while the degree of cross-linking was examined with TGA-DA. The smaller pores and greatest swelling were found in hydrogels containing 10 wt% of GA. However, only the hydrogels with GA at 2, 4 and 6 wt% displayed viable cells, indicating their in vitro cyto- compatibility. The rheological studies showed that the values of the loss and storage modules in the hydrogels

KW - CS, chitosan; GA, glutaraldehyde; DNP, 2,4-dinitrophenylhydrazine; wt%, percentage of weight; UV-vis, ultraviolet-visible spectroscopy; FT-IR, Fourier-transform infrared spectroscopy; SEM, scanning electron microcopy; DA-TGA, derivate thermogravimetric an

U2 - doi.org/10.1016/j.colsurfa.2019.123658

DO - doi.org/10.1016/j.colsurfa.2019.123658

M3 - Artículo

SN - 0927-7757

VL - 579

SP - 1

EP - 15

JO - Colloids and Surfaces A: Physicochemical and Engineering Aspects

JF - Colloids and Surfaces A: Physicochemical and Engineering Aspects

IS - 123658

M1 - 123658

ER -