TY - JOUR
T1 - Superoxide dismutase activity in juvenile Litopenaeus vannamei and Nodipecten subnodosus exposed to the toxic dinoflagellate Prorocentrum lima
AU - Campa-Córdova, Angel I.
AU - Núñez-Vázquez, Erick J.
AU - Luna-González, Antonio
AU - Romero-Geraldo, María J.
AU - Ascencio, Felipe
N1 - Funding Information:
We thank Viridiana Peraza-Gómez and Alejandra Heredia-Tapia for technical support and the editor from CIBNOR for improving the English text. This work was funded by a grant from Centro de Investigaciones Biológicas del Noroeste (CIBNOR Grant AC1.19).
PY - 2009/4
Y1 - 2009/4
N2 - The toxic effect of the dinoflagellate Prorocentrum lima on juvenile American whiteleg shrimp Litopenaeus vannamei and giant lion-paw scallop Nodipecten subnodosus was evaluated. Organisms were exposed to three densities (500, 2000, or 5000 cells mL- 1), superoxide dismutase activity and soluble protein in the hepatopancreas and muscle were determined at 1, 6, 24 and 48 h after challenge. Shrimp exposed at 5000 cells mL- 1 significantly increased SOD activity in the hepatopancreas at 1 h post-challenge, whereas enzymatic activity in muscle significantly increased at 24 h at all densities. Scallops exposed to 500 and 2000 cells mL- 1 showed significant SOD activity increase in hepatopancreas at 24 and 12 h, respectively. Mortality at 48 h was 100% in scallops exposed to 5000 cells mL- 1. Shrimp showed higher levels of SOD activity than scallops. Soluble protein content in the shrimp hepatopancreas was significantly higher at densities of 500 and 2000 cells mL- 1 at 6 and 1 h, respectively. Soluble protein content in the scallop hepatopancreas was higher than control values at 1 h after challenge. In this study, 500 cells mL- 1 was enough to trigger SOD activity in two benthic species exposed to the toxic dinoflagellate P. lima.
AB - The toxic effect of the dinoflagellate Prorocentrum lima on juvenile American whiteleg shrimp Litopenaeus vannamei and giant lion-paw scallop Nodipecten subnodosus was evaluated. Organisms were exposed to three densities (500, 2000, or 5000 cells mL- 1), superoxide dismutase activity and soluble protein in the hepatopancreas and muscle were determined at 1, 6, 24 and 48 h after challenge. Shrimp exposed at 5000 cells mL- 1 significantly increased SOD activity in the hepatopancreas at 1 h post-challenge, whereas enzymatic activity in muscle significantly increased at 24 h at all densities. Scallops exposed to 500 and 2000 cells mL- 1 showed significant SOD activity increase in hepatopancreas at 24 and 12 h, respectively. Mortality at 48 h was 100% in scallops exposed to 5000 cells mL- 1. Shrimp showed higher levels of SOD activity than scallops. Soluble protein content in the shrimp hepatopancreas was significantly higher at densities of 500 and 2000 cells mL- 1 at 6 and 1 h, respectively. Soluble protein content in the scallop hepatopancreas was higher than control values at 1 h after challenge. In this study, 500 cells mL- 1 was enough to trigger SOD activity in two benthic species exposed to the toxic dinoflagellate P. lima.
KW - Antioxidant enzymes
KW - Litopenaeus vannamei
KW - Nodipecten subnodosus
KW - Prorocentrum lima
KW - SOD
KW - Toxicity
UR - http://www.scopus.com/inward/record.url?scp=60949094083&partnerID=8YFLogxK
U2 - 10.1016/j.cbpc.2008.08.006
DO - 10.1016/j.cbpc.2008.08.006
M3 - Artículo
C2 - 18786656
AN - SCOPUS:60949094083
SN - 1532-0456
VL - 149
SP - 317
EP - 322
JO - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
JF - Comparative Biochemistry and Physiology - C Toxicology and Pharmacology
IS - 3
ER -