TY - JOUR
T1 - Staphylococcus epidermidis with the icaA-/icaD-/IS256- genotype and protein or protein/extracellular-DNA biofilm is frequent in ocular infections
AU - Juárez-Verdayes, Marco A.
AU - Ramón-Peréz, Miriam L.
AU - Flores-Páez, Luis A.
AU - Camarillo-Márquez, Omar
AU - Zenteno, Juan C.
AU - Jan-Roblero, Janet
AU - Cancino-Diaz, Mario E.
AU - Cancino-Diaz, Juan C.
PY - 2013
Y1 - 2013
N2 - In ocular infections (OIs) caused by Staphylococcus epidermidis, biofilms composed mainly of poly-N-acetylglucosamine (PNAG) have been widely studied, but PNAG-independent biofilms have not. Therefore, we searched for a relationship between the ica operon (involved in PNAG-biofilm) and the biochemical composition of biofilms in isolates from OI. Isolates from OI (n = 62), from healthy conjunctiva (HC; n = 45) and from healthy skin (HS; n = 53), were used to detect icaA and icaD genes, and the insertion sequence 256 (IS256) using PCR. The compositions of the biofilms were determined by treatment with NaIO4, proteinase K and DNase I. Multilocus sequence typing (MLST) was performed to characterize the isolates, and the expression of aap and embp genes was determined by real-time qPCR. A strong relationship between the icaA-/icaD-/IS256- genotype and protein or protein/extracellular DNA (eDNA)-biofilm composition was found in the isolates from OI (53.6 %), whereas the icaA+/icaD+/IS256- genotype and carbohydrate-biofilm was most prevalent in isolates from HC (25 %) and HS (25 %). Isolates with an icaA-/icaD-/IS256- genotype and protein-biofilm phenotype were predominantly of the ST2 lineage, while carbohydrate-biofilm-producing strains were mainly of the ST9 lineage. The protein-biofilm-producing strains had higher expression levels of aap gene than carbohydrate-biofilm-producing strains; while embp gene did not have the same pattern of expression. These results suggest that S. epidermidis strains with icaA-/icaD-/IS256- genotype and protein or protein/eDNA-biofilms have a stronger ability to establish in the eye than S. epidermidis strains with icaA+/icaD+/IS256- genotype and PNAG-biofilms.
AB - In ocular infections (OIs) caused by Staphylococcus epidermidis, biofilms composed mainly of poly-N-acetylglucosamine (PNAG) have been widely studied, but PNAG-independent biofilms have not. Therefore, we searched for a relationship between the ica operon (involved in PNAG-biofilm) and the biochemical composition of biofilms in isolates from OI. Isolates from OI (n = 62), from healthy conjunctiva (HC; n = 45) and from healthy skin (HS; n = 53), were used to detect icaA and icaD genes, and the insertion sequence 256 (IS256) using PCR. The compositions of the biofilms were determined by treatment with NaIO4, proteinase K and DNase I. Multilocus sequence typing (MLST) was performed to characterize the isolates, and the expression of aap and embp genes was determined by real-time qPCR. A strong relationship between the icaA-/icaD-/IS256- genotype and protein or protein/extracellular DNA (eDNA)-biofilm composition was found in the isolates from OI (53.6 %), whereas the icaA+/icaD+/IS256- genotype and carbohydrate-biofilm was most prevalent in isolates from HC (25 %) and HS (25 %). Isolates with an icaA-/icaD-/IS256- genotype and protein-biofilm phenotype were predominantly of the ST2 lineage, while carbohydrate-biofilm-producing strains were mainly of the ST9 lineage. The protein-biofilm-producing strains had higher expression levels of aap gene than carbohydrate-biofilm-producing strains; while embp gene did not have the same pattern of expression. These results suggest that S. epidermidis strains with icaA-/icaD-/IS256- genotype and protein or protein/eDNA-biofilms have a stronger ability to establish in the eye than S. epidermidis strains with icaA+/icaD+/IS256- genotype and PNAG-biofilms.
UR - http://www.scopus.com/inward/record.url?scp=84884480593&partnerID=8YFLogxK
U2 - 10.1099/jmm.0.055210-0
DO - 10.1099/jmm.0.055210-0
M3 - Artículo
C2 - 23861297
SN - 0022-2615
VL - 62
SP - 1579
EP - 1587
JO - Journal of Medical Microbiology
JF - Journal of Medical Microbiology
IS - PART10
ER -