TY - JOUR
T1 - Spinal nerve ligation reduces nitric oxide synthase activity and expression
T2 - Effect of resveratrol
AU - Pérez-Severiano, Francisca
AU - Bermúdez-Ocaña, Deysi Y.
AU - López-Sánchez, Pedro
AU - Ríos, Camilo
AU - Granados-Soto, Vinicio
N1 - Funding Information:
Authors greatly appreciate the technical assistance of Paula Vergara and Guadalupe C. Vidal-Cantú. Deysi Y. Bermúdez-Ocaña is a CONACYT fellow. This work is part of the Ph.D. dissertation of Deysi Y. Bermúdez-Ocaña. Partially supported by Conacyt grants 46217 (PLS), 61327 (FPS) and 59879-M (VGS).
PY - 2008/10
Y1 - 2008/10
N2 - The effect of resveratrol on activity and expression of nitric oxide synthase (NOS) in the spinal cord of neuropathic rats was assessed. Spinal nerve ligation produced tactile allodynia along with a reduction of catalytic activity of the constitutive Ca2+-dependent NOS (eNOS and nNOS isoforms) in the ipsilateral dorsal horn, but not contralateral dorsal or ipsilateral or contralateral ventral, spinal cord at 1, 5, 10 and 15 days after surgery compared to naïve and sham-operated animals. Nerve ligation also induced a reduction of nNOS expression in the ipsilateral dorsal horn spinal cord at 10 and 15 days after surgery. Intrathecal resveratrol reduced allodynia and reversed the reduction of constitutive Ca2+-dependent NOS activity in the ipsilateral dorsal spinal cord. Moreover, resveratrol significantly reversed the reduction of nNOS expression in the ipsilateral dorsal horn spinal cord. Results show that spinal nerve ligation leads to development of tactile allodynia along with a reduction in constitutive Ca2+-dependent NOS activity and nNOS isoform expression in the ipsilateral dorsal horn. Data suggest that resveratrol may reduce tactile allodynia in neuropathic rats by restoring altered NOS activity and expression.
AB - The effect of resveratrol on activity and expression of nitric oxide synthase (NOS) in the spinal cord of neuropathic rats was assessed. Spinal nerve ligation produced tactile allodynia along with a reduction of catalytic activity of the constitutive Ca2+-dependent NOS (eNOS and nNOS isoforms) in the ipsilateral dorsal horn, but not contralateral dorsal or ipsilateral or contralateral ventral, spinal cord at 1, 5, 10 and 15 days after surgery compared to naïve and sham-operated animals. Nerve ligation also induced a reduction of nNOS expression in the ipsilateral dorsal horn spinal cord at 10 and 15 days after surgery. Intrathecal resveratrol reduced allodynia and reversed the reduction of constitutive Ca2+-dependent NOS activity in the ipsilateral dorsal spinal cord. Moreover, resveratrol significantly reversed the reduction of nNOS expression in the ipsilateral dorsal horn spinal cord. Results show that spinal nerve ligation leads to development of tactile allodynia along with a reduction in constitutive Ca2+-dependent NOS activity and nNOS isoform expression in the ipsilateral dorsal horn. Data suggest that resveratrol may reduce tactile allodynia in neuropathic rats by restoring altered NOS activity and expression.
KW - NOS activity
KW - Neuropathic pain
KW - Resveratrol
KW - Tactile allodynia
KW - nNOS expression
UR - http://www.scopus.com/inward/record.url?scp=48749111330&partnerID=8YFLogxK
U2 - 10.1016/j.pbb.2008.05.024
DO - 10.1016/j.pbb.2008.05.024
M3 - Artículo
C2 - 18582495
SN - 0091-3057
VL - 90
SP - 742
EP - 747
JO - Pharmacology Biochemistry and Behavior
JF - Pharmacology Biochemistry and Behavior
IS - 4
ER -