TY - JOUR
T1 - Spermidine-condensed DNA and cone-shaped lipids improve delivery and expression of exogenous DNA transfer by liposomes
AU - Ibáñez, Miguel
PY - 1996
Y1 - 1996
N2 - A new liposome system containing spermidine-condensed DNA and negative cone-forming lipids designed to improve gene delivery and expression is described. The compacted nature of condensed DNA forms permitted a higher extent of encapsulation of DNA in liposomes. These vesicles contained fusogenic cone-shaped lipids to increase fusion between liposomes and membranes to enhance the amount of DNA delivery into the cells. In addition, the insensitivity of condensed DNA forms to endonucleases and restriction enzymes, as well as their higher activity in both replication and transcription, improve foreign DNA expression. These improvements in condensed DNA encapsulation in liposomes, transfer into the cells, and DNA expression increase the number of transfected cells and produce a higher level of gene expression in most transfected cells. This is reflected in the 60-fold cell culture transfection increase compared with traditional liposome transfection systems. This liposome system does not cause any apparent damage to the transfected cells; furthermore, the liposomes are small, 400500 nm, and have negative surface charges that can prolonge their circulation half-lives in vivo, permitting their use for in vivo gene therapy applications.
AB - A new liposome system containing spermidine-condensed DNA and negative cone-forming lipids designed to improve gene delivery and expression is described. The compacted nature of condensed DNA forms permitted a higher extent of encapsulation of DNA in liposomes. These vesicles contained fusogenic cone-shaped lipids to increase fusion between liposomes and membranes to enhance the amount of DNA delivery into the cells. In addition, the insensitivity of condensed DNA forms to endonucleases and restriction enzymes, as well as their higher activity in both replication and transcription, improve foreign DNA expression. These improvements in condensed DNA encapsulation in liposomes, transfer into the cells, and DNA expression increase the number of transfected cells and produce a higher level of gene expression in most transfected cells. This is reflected in the 60-fold cell culture transfection increase compared with traditional liposome transfection systems. This liposome system does not cause any apparent damage to the transfected cells; furthermore, the liposomes are small, 400500 nm, and have negative surface charges that can prolonge their circulation half-lives in vivo, permitting their use for in vivo gene therapy applications.
KW - Cone-shaped lipids
KW - Gene transfer
KW - Liposome
KW - Spermidine-condensed DNA
UR - http://www.scopus.com/inward/record.url?scp=0030340799&partnerID=8YFLogxK
U2 - 10.1139/o96-068
DO - 10.1139/o96-068
M3 - Artículo
SN - 0829-8211
VL - 74
SP - 633
EP - 643
JO - Biochemistry and Cell Biology
JF - Biochemistry and Cell Biology
IS - 5
ER -