TY - JOUR
T1 - SLC41A1 is the only magnesium responsive gene significantly overexpressed in placentas of preeclamptic women
AU - Kolisek, Martin
AU - Galaviz-Hernández, Carlos
AU - Vázquez-Alaniz, Fernando
AU - Sponder, Gerhard
AU - Javaid, Saba
AU - Kurth, Katharina
AU - Nestler, Axel
AU - Rodríguez-Moran, Martha
AU - Verlohren, Stefan
AU - Guerrero-Romero, Fernando
AU - Aschenbach, Jörg Rudolf
AU - Vormann, Jürgen
N1 - Funding Information:
This project was supported by MEX09/001 issued by BMBF to JV (Germany) and by the project FONSEC 2009-01-111870 (CONACyT, Mexico) to CGH.
PY - 2013/11
Y1 - 2013/11
N2 - Objective: To examine expression profile of magnesium responsive genes (MRGs) in placentas of normoevolutive and preeclamptic women. Methods: The expression profiles of MRGs were determined in placentas of normoevolutive (N = 26) and preeclamptic (N = 25) women by RT-qPCR. Results: Among all tested MRGs (9) only SLC41A1 (encoding for Na+/Mg2+ exchanger) was significantly overexpressed in ∼54.2% of preeclamptic (n = 24) and in ∼9.5% of normoevolutive (n = 21) specimens. On average, SLC41A1 was overexpressed sixfold in the preeclamptic group. Presence of SLC41A1 in placentas was confirmed by Western blot analysis. Conclusion. SLC41A1 is significantly overexpressed in nearly 55% of preeclamptic placentas. This may indicate a direct contribution of changed Mg homeostasis in the development of preeclampsia.
AB - Objective: To examine expression profile of magnesium responsive genes (MRGs) in placentas of normoevolutive and preeclamptic women. Methods: The expression profiles of MRGs were determined in placentas of normoevolutive (N = 26) and preeclamptic (N = 25) women by RT-qPCR. Results: Among all tested MRGs (9) only SLC41A1 (encoding for Na+/Mg2+ exchanger) was significantly overexpressed in ∼54.2% of preeclamptic (n = 24) and in ∼9.5% of normoevolutive (n = 21) specimens. On average, SLC41A1 was overexpressed sixfold in the preeclamptic group. Presence of SLC41A1 in placentas was confirmed by Western blot analysis. Conclusion. SLC41A1 is significantly overexpressed in nearly 55% of preeclamptic placentas. This may indicate a direct contribution of changed Mg homeostasis in the development of preeclampsia.
KW - Magnesium
KW - Na/Mg exchanger
KW - Overexpression
KW - Preeclampsia
KW - RT-qPCR.
UR - http://www.scopus.com/inward/record.url?scp=84881560223&partnerID=8YFLogxK
U2 - 10.3109/10641955.2013.810237
DO - 10.3109/10641955.2013.810237
M3 - Artículo
SN - 1064-1955
VL - 32
SP - 378
EP - 389
JO - Hypertension in Pregnancy
JF - Hypertension in Pregnancy
IS - 4
ER -