TY - JOUR
T1 - Simple Method for the Determination of Cefaclor in Human Plasma Samples by HPLC
AU - Granados-Soto, V.
AU - Aguilar-Cota, M. E.
AU - Reyes-Garcia, G.
AU - Medina-Santillán, R.
AU - Flores-Murrieta, F. J.
N1 - Funding Information:
The authors greatly appreciate the bibliographic assistance of Hector Vázquez. V. Granados-Soto is a recipient of a sabbatical fellowship from CONACYT.
PY - 2003
Y1 - 2003
N2 - A rapid and simple method for determination of cefaclor in human plasma samples, by high-performance liquid chromatography (HPLC), was developed. This method includes a deproteinization with perchloric acid as extraction procedure. Plasma extracts were analyzed on a reversed phase column eluted with a mixture of acetonitrile and 0.01 M sodium dihydrogen phosphate solution, adjusted to pH 6.5 with potassium hydroxide (7: 93, v/v), and detected by absorbance at 260 nm. Retention times for cefaclor and the internal standard were 5.2 and 7.2 min, respectively. The method was linear in the range of 0.5-30 μg/mL and the detection limit of the method was 100 ng/mL. This method was useful and suitable for determination of cefaclor after oral administration in healthy volunteers, and could be used for bioavailability and bioequivalence studies of the drug.
AB - A rapid and simple method for determination of cefaclor in human plasma samples, by high-performance liquid chromatography (HPLC), was developed. This method includes a deproteinization with perchloric acid as extraction procedure. Plasma extracts were analyzed on a reversed phase column eluted with a mixture of acetonitrile and 0.01 M sodium dihydrogen phosphate solution, adjusted to pH 6.5 with potassium hydroxide (7: 93, v/v), and detected by absorbance at 260 nm. Retention times for cefaclor and the internal standard were 5.2 and 7.2 min, respectively. The method was linear in the range of 0.5-30 μg/mL and the detection limit of the method was 100 ng/mL. This method was useful and suitable for determination of cefaclor after oral administration in healthy volunteers, and could be used for bioavailability and bioequivalence studies of the drug.
KW - Cefaclor
KW - HPLC
KW - Human plasma
KW - Pharmacokinetics
UR - http://www.scopus.com/inward/record.url?scp=0345687501&partnerID=8YFLogxK
U2 - 10.1081/JLC-120025526
DO - 10.1081/JLC-120025526
M3 - Artículo
SN - 1082-6076
VL - 26
SP - 3315
EP - 3323
JO - Journal of Liquid Chromatography and Related Technologies
JF - Journal of Liquid Chromatography and Related Technologies
IS - 19
ER -