TY - JOUR
T1 - Severity of SARS-CoV-2 infection is linked to double-negative (CD27− IgD−) B cell subset numbers
AU - Cervantes-Díaz, Rodrigo
AU - Sosa-Hernández, Víctor Andrés
AU - Torres-Ruíz, Jiram
AU - Romero-Ramírez, Sandra
AU - Cañez-Hernández, Mariana
AU - Pérez-Fragoso, Alfredo
AU - Páez-Franco, José C.
AU - Meza-Sánchez, David E.
AU - Pescador-Rojas, Miriam
AU - Sosa-Hernández, Víctor Adrián
AU - Gómez-Martín, Diana
AU - Maravillas-Montero, José L.
N1 - Publisher Copyright:
© 2021, The Author(s), under exclusive licence to Springer Nature Switzerland AG.
PY - 2022/1
Y1 - 2022/1
N2 - Objectives: The role of B cells in COVID‐19, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Here, we describe the novel landscape of circulating double-negative (DN) CD27− IgD− B cells in COVID‐19 patients, representing a group of atypical and neglected subpopulations of this cell lineage. Methods: Using multiparametric flow cytometry, we determined DN B cell subset amounts from 91 COVID-19 patients, correlated those with cytokines, clinical and laboratory parameters, and segregated them by principal components analysis. Results: We detected significant increments in the DN2 and DN3 B cell subsets, while we found a relevant decrease in the DN1 B cell subpopulation, according to disease severity and patient outcomes. These DN cell numbers also appeared to correlate with pro- or anti-inflammatory signatures, respectively, and contributed to the segregation of the patients into disease severity groups. Conclusion: This study provides insights into DN B cell subsets’ potential role in immune responses against SARS‐CoV‐2, particularly linked to the severity of COVID‐19.
AB - Objectives: The role of B cells in COVID‐19, beyond the production of specific antibodies against SARS-CoV-2, is still not well understood. Here, we describe the novel landscape of circulating double-negative (DN) CD27− IgD− B cells in COVID‐19 patients, representing a group of atypical and neglected subpopulations of this cell lineage. Methods: Using multiparametric flow cytometry, we determined DN B cell subset amounts from 91 COVID-19 patients, correlated those with cytokines, clinical and laboratory parameters, and segregated them by principal components analysis. Results: We detected significant increments in the DN2 and DN3 B cell subsets, while we found a relevant decrease in the DN1 B cell subpopulation, according to disease severity and patient outcomes. These DN cell numbers also appeared to correlate with pro- or anti-inflammatory signatures, respectively, and contributed to the segregation of the patients into disease severity groups. Conclusion: This study provides insights into DN B cell subsets’ potential role in immune responses against SARS‐CoV‐2, particularly linked to the severity of COVID‐19.
KW - B cell
KW - COVID-19
KW - DN B cell
KW - Inflammation
UR - http://www.scopus.com/inward/record.url?scp=85120340213&partnerID=8YFLogxK
U2 - 10.1007/s00011-021-01525-3
DO - 10.1007/s00011-021-01525-3
M3 - Artículo
C2 - 34850243
AN - SCOPUS:85120340213
SN - 1023-3830
VL - 71
SP - 131
EP - 140
JO - Inflammation Research
JF - Inflammation Research
IS - 1
ER -