TY - JOUR
T1 - Screening for potential probiotic bacteria to reduce prevalence of WSSV and IHHNV in whiteleg shrimp (Litopenaeus vannamei) under experimental conditions
AU - Leyva-Madrigal, Karla Y.
AU - Luna-González, Antonio
AU - Escobedo-Bonilla, César M.
AU - Fierro-Coronado, Jesús A.
AU - Maldonado-Mendoza, Ignacio E.
N1 - Funding Information:
Authors are grateful to Consejo Estatal de Ciencia y Tecnología del Estado de Sinaloa (CECyT-Sinaloa) and the Secretaría de Investigación y Posgrado del Instituto Politécnico Nacional (SIP-IPN) for financial support. Karla Y. Leyva Madrigal acknowledges Consejo Nacional de Ciencia y Tecnología (CONACYT, México) and SIP-IPN for M.Sc. fellowships.
PY - 2011/12/21
Y1 - 2011/12/21
N2 - This study evaluated the effect of Pediococcus pentosaceus and Staphylococcus hemolyticus as probiotics in whiteleg shrimp Litopenaeus vannamei naturally infected with WSSV and IHHNV. All bacteria were isolated from the gut of wild brown shrimp (Farfantepenaeus californiensis). Presumptive lactic acid bacteria were characterized for hemolytic and enzymatic activity, hydrophobicity, growth, and molecular identification. Two mixtures of four isolates were tested and their effect measured on the hemocyte number, survival, and prevalence of WSSV and IHHNV. Each mixture was applied at two different concentrations in a 15-day bioassay with shrimp naturally infected with WSSV and IHHNV as determined by single and/or nested PCR. In the treated animals total hemocyte count and survival were similar to control group. All shrimp fed with bacterial mixtures showed a decrease in the prevalence of WSSV but not IHHNV. The results obtained in this preliminary study revealed a protective effect of the two bacterial mixtures against WSSV latent infections.
AB - This study evaluated the effect of Pediococcus pentosaceus and Staphylococcus hemolyticus as probiotics in whiteleg shrimp Litopenaeus vannamei naturally infected with WSSV and IHHNV. All bacteria were isolated from the gut of wild brown shrimp (Farfantepenaeus californiensis). Presumptive lactic acid bacteria were characterized for hemolytic and enzymatic activity, hydrophobicity, growth, and molecular identification. Two mixtures of four isolates were tested and their effect measured on the hemocyte number, survival, and prevalence of WSSV and IHHNV. Each mixture was applied at two different concentrations in a 15-day bioassay with shrimp naturally infected with WSSV and IHHNV as determined by single and/or nested PCR. In the treated animals total hemocyte count and survival were similar to control group. All shrimp fed with bacterial mixtures showed a decrease in the prevalence of WSSV but not IHHNV. The results obtained in this preliminary study revealed a protective effect of the two bacterial mixtures against WSSV latent infections.
KW - IHHNV
KW - Litopenaeus vannamei
KW - Pediococcus
KW - Staphylococcus
KW - WSSV
UR - http://www.scopus.com/inward/record.url?scp=83655167190&partnerID=8YFLogxK
U2 - 10.1016/j.aquaculture.2011.09.033
DO - 10.1016/j.aquaculture.2011.09.033
M3 - Artículo
SN - 0044-8486
VL - 322-323
SP - 16
EP - 22
JO - Aquaculture
JF - Aquaculture
ER -