TY - JOUR
T1 - Scorpion antivenom effect of micropropagated Aristolochia elegans
AU - Izquierdo, Alejandro Mora
AU - Zapata, Elsa Ventura
AU - Jiménez-Ferrer, J. Enrique
AU - Muñoz, Crescencio Bazaldúa
AU - Aparicio, Antonio Jiménez
AU - Torres, Kalina Bermdez
AU - Torres, Lidia Osuna
PY - 2010/8/1
Y1 - 2010/8/1
N2 - Aristolochia elegans Mast. (Aristolochiaceae) has been used to treat scorpion envenoming in Mexican traditional medicine. In vitro studies of the pharmacological activity of raw extracts from A. elegans roots have shown activity against scorpion bite. The aim of the present study was to determine for the first time the antagonistic effect of hexane and methanol extracts of the aerial parts and roots from micropropagated A. elegans plants in a model of isolated guinea-pig ileum contracted by scorpion bite. Results showed that the methanol extracts of aerial organs (74%) and roots (65%) of micropropagated plants have a similar antitoxin activity against scorpion poisoning to hexane extracts of wild plants (65%). These results suggest that using methanol extracts from the micropropagated plant material instead of wild plant root extracts from A. elegans is an alternative for treatment against scorpion bite symptoms, and will contribute to the conservation of this medicinal species.
AB - Aristolochia elegans Mast. (Aristolochiaceae) has been used to treat scorpion envenoming in Mexican traditional medicine. In vitro studies of the pharmacological activity of raw extracts from A. elegans roots have shown activity against scorpion bite. The aim of the present study was to determine for the first time the antagonistic effect of hexane and methanol extracts of the aerial parts and roots from micropropagated A. elegans plants in a model of isolated guinea-pig ileum contracted by scorpion bite. Results showed that the methanol extracts of aerial organs (74%) and roots (65%) of micropropagated plants have a similar antitoxin activity against scorpion poisoning to hexane extracts of wild plants (65%). These results suggest that using methanol extracts from the micropropagated plant material instead of wild plant root extracts from A. elegans is an alternative for treatment against scorpion bite symptoms, and will contribute to the conservation of this medicinal species.
KW - Centruroides limpidus limpidus Karsch
KW - Hexane extract
KW - Isolated guinea-pig ileum model
KW - Medicinal plant
KW - Methanol extract
KW - Relaxation test
UR - http://www.scopus.com/inward/record.url?scp=77955187242&partnerID=8YFLogxK
U2 - 10.3109/13880200903311110
DO - 10.3109/13880200903311110
M3 - Artículo
SN - 1388-0209
VL - 48
SP - 891
EP - 896
JO - Pharmaceutical Biology
JF - Pharmaceutical Biology
IS - 8
ER -