TY - JOUR
T1 - Dextran synthesis by native sugarcane microorganisms
AU - Castilla-Marroquín, J. D.
AU - Hernández-Martínez, R.
AU - Debernardi-De la Vequia, H.
AU - Ríos-Corripio, M. A.
AU - Hernández-Rosas, J.
AU - Rojas-López, M.
AU - Hernández-Rosas, F.
N1 - Publisher Copyright:
© 2020, Universidad Autonoma Metropolitana Iztapalapa. All rights reserved.
PY - 2020
Y1 - 2020
N2 - The sugarcane agri-food industry boosts the Mexican economy in producing regions. Basing its relevance in sugar production yields. However, by-products are not widely exploited leaving an opportunity for diversification. In this study, three microorganism isolates (A, B, and C) were obtained from sugarcane kefir; the morphology of isolates B and C corresponded to the lactic acid bacterial genus Leuconostoc. Thus, we examined the potential for these isolates to produce EPSs, like dextran, a molecule with applications in pharmaceuticals, industrials, and foods. The experiment was performed adjusting the active culture concentration to 1 ×106colony-forming units (CFU)/ml, the culture was maintained at 37°C in agitation at 150 rpm. The obtained EPSs were purified by ethanol and cold acetone precipitation. The results showed that B and C bacterial isolates had the capacity to produce EPSs (14 g/L for isolate B and 32 g/L for isolate C) after 24 h. Fourier-transform infrared spectroscopy (FT-IR) characterization indicated that the EPS was dextran. Further, the produced biopolymer had high solubility in water, avoided freezing at -4°C, and boiled at 85°C.
AB - The sugarcane agri-food industry boosts the Mexican economy in producing regions. Basing its relevance in sugar production yields. However, by-products are not widely exploited leaving an opportunity for diversification. In this study, three microorganism isolates (A, B, and C) were obtained from sugarcane kefir; the morphology of isolates B and C corresponded to the lactic acid bacterial genus Leuconostoc. Thus, we examined the potential for these isolates to produce EPSs, like dextran, a molecule with applications in pharmaceuticals, industrials, and foods. The experiment was performed adjusting the active culture concentration to 1 ×106colony-forming units (CFU)/ml, the culture was maintained at 37°C in agitation at 150 rpm. The obtained EPSs were purified by ethanol and cold acetone precipitation. The results showed that B and C bacterial isolates had the capacity to produce EPSs (14 g/L for isolate B and 32 g/L for isolate C) after 24 h. Fourier-transform infrared spectroscopy (FT-IR) characterization indicated that the EPS was dextran. Further, the produced biopolymer had high solubility in water, avoided freezing at -4°C, and boiled at 85°C.
KW - Biopolymers
KW - Dextran
KW - Dextransucrase
KW - Extracellular polymeric substances
KW - Sucrose
UR - http://www.scopus.com/inward/record.url?scp=85090641803&partnerID=8YFLogxK
U2 - 10.24275/rmiq/Bio1793
DO - 10.24275/rmiq/Bio1793
M3 - Artículo
AN - SCOPUS:85090641803
SN - 1665-2738
VL - 19
SP - 177
EP - 185
JO - Revista Mexicana de Ingeniera Quimica
JF - Revista Mexicana de Ingeniera Quimica
ER -