TY - JOUR
T1 - Roles of multiple promoters in transcription of ribosomal DNA
T2 - Effects of growth conditions on precursor rRNA synthesis in mycobacteria
AU - Gonzalez-y-Merchand, J. A.
AU - Colston, M. J.
AU - Cox, R. A.
PY - 1998
Y1 - 1998
N2 - The roles of multiple promoters in the synthesis of rRNA under different conditions of growth were investigated, using two mycobacterial species as model organisms. When Mycobacterium smegmatis was grown under optimal conditions, its two rRNA operons contributed equally, with two promoters, one from each operon, being responsible for most transcripts. In stationary- phase growth or balanced growth under carbon starvation conditions, one operon (rrnA(f)) dominated and its three promoters contributed more equally to the generation of transcripts. Mycobacterium tuberculosis has a single operon with two promoters, one of which generated 80% of transcripts, at all stages of growth. We infer that each promoter functions independently according to its intrinsic strength when cells are growing slowly so that one operon with three promoters is roughly equivalent to three operons with one promoter; at high growth rates, occlusion effects reduce the efficiency of multiple promoters to that of a single promoter.
AB - The roles of multiple promoters in the synthesis of rRNA under different conditions of growth were investigated, using two mycobacterial species as model organisms. When Mycobacterium smegmatis was grown under optimal conditions, its two rRNA operons contributed equally, with two promoters, one from each operon, being responsible for most transcripts. In stationary- phase growth or balanced growth under carbon starvation conditions, one operon (rrnA(f)) dominated and its three promoters contributed more equally to the generation of transcripts. Mycobacterium tuberculosis has a single operon with two promoters, one of which generated 80% of transcripts, at all stages of growth. We infer that each promoter functions independently according to its intrinsic strength when cells are growing slowly so that one operon with three promoters is roughly equivalent to three operons with one promoter; at high growth rates, occlusion effects reduce the efficiency of multiple promoters to that of a single promoter.
UR - http://www.scopus.com/inward/record.url?scp=0031740085&partnerID=8YFLogxK
U2 - 10.1128/jb.180.21.5756-5761.1998
DO - 10.1128/jb.180.21.5756-5761.1998
M3 - Artículo
SN - 0021-9193
VL - 180
SP - 5756
EP - 5761
JO - Journal of Bacteriology
JF - Journal of Bacteriology
IS - 21
ER -