TY - JOUR
T1 - Role of TRPV1 and ASIC3 in formalin-induced secondary allodynia and hyperalgesia
AU - Martínez-Rojas, Vladimir A.
AU - Barragán-Iglesias, Paulino
AU - Rocha-González, Héctor I.
AU - Murbartián, Janet
AU - Granados-Soto, Vinicio
N1 - Funding Information:
Vladimir A. Martínez-Rojas and Paulino Barragán-Iglesias are Conacyt fellows. This work is part of the M.Sc. dissertation of Vladimir A. Martínez-Rojas. Partially supported by Conacyt CB-2012/179294 (VG-S), 154880 (HIR-G) and ICyTDF 332-2011 (JM) grants.
PY - 2014/12
Y1 - 2014/12
N2 - Background In the present study we determined the role of transient receptor potential V1 channel (TRPV1) and acid-sensing ion channel 3 (ASIC3) in chronic nociception. Methods 1% formalin was used to produce long-lasting secondary allodynia and hyperalgesia in rats. Western blot was used to determine TRPV1 and ASIC3 expression in dorsal root ganglia. Results Peripheral ipsilateral, but not contralateral, pre-treatment (-10 min) with the TRPV1 receptor antagonists capsazepine (0.03-0.3 μM/paw) and A-784168 (0.01-1 μM/paw) prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in the ipsilateral and contralateral paws. Likewise, peripheral ipsilateral, but not contralateral, pre-treatment with the non-selective and selective ASIC3 blocker benzamil (0.1-10 μM/paw) and APETx2 (0.02-2 μM/paw), respectively, prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. Peripheral ipsilateral post-treatment (day 6 after formalin injection) with capsazepine (0.03-0.3 μM/paw) and A-784168 (0.01-1 μM/paw) reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. In addition, peripheral ipsilateral post-treatment with benzamil (0.1-10 μM/paw) and APETx2 (0.02-2 μM/paw), respectively, reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. TRPV1 and ASIC3 proteins were expressed in dorsal root ganglion in normal conditions, and 1% formalin injection increased expression of both proteins in this location at 1 and 6 days compared to naive rats. Conclusions Data suggest that TRPV1 and ASIC3 participate in the development and maintenance of long-lasting secondary allodynia and hyperalgesia induced by formalin in rats. The use of TRPV1 and ASIC3 antagonists by peripheral administration could prove useful to treat chronic pain.
AB - Background In the present study we determined the role of transient receptor potential V1 channel (TRPV1) and acid-sensing ion channel 3 (ASIC3) in chronic nociception. Methods 1% formalin was used to produce long-lasting secondary allodynia and hyperalgesia in rats. Western blot was used to determine TRPV1 and ASIC3 expression in dorsal root ganglia. Results Peripheral ipsilateral, but not contralateral, pre-treatment (-10 min) with the TRPV1 receptor antagonists capsazepine (0.03-0.3 μM/paw) and A-784168 (0.01-1 μM/paw) prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in the ipsilateral and contralateral paws. Likewise, peripheral ipsilateral, but not contralateral, pre-treatment with the non-selective and selective ASIC3 blocker benzamil (0.1-10 μM/paw) and APETx2 (0.02-2 μM/paw), respectively, prevented 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. Peripheral ipsilateral post-treatment (day 6 after formalin injection) with capsazepine (0.03-0.3 μM/paw) and A-784168 (0.01-1 μM/paw) reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. In addition, peripheral ipsilateral post-treatment with benzamil (0.1-10 μM/paw) and APETx2 (0.02-2 μM/paw), respectively, reversed 1% formalin-induced secondary mechanical allodynia and hyperalgesia in both paws. TRPV1 and ASIC3 proteins were expressed in dorsal root ganglion in normal conditions, and 1% formalin injection increased expression of both proteins in this location at 1 and 6 days compared to naive rats. Conclusions Data suggest that TRPV1 and ASIC3 participate in the development and maintenance of long-lasting secondary allodynia and hyperalgesia induced by formalin in rats. The use of TRPV1 and ASIC3 antagonists by peripheral administration could prove useful to treat chronic pain.
KW - ASIC3
KW - Chronic pain
KW - Secondary allodynia
KW - Secondary hyperalgesia
KW - TRPV1
UR - http://www.scopus.com/inward/record.url?scp=84905821528&partnerID=8YFLogxK
U2 - 10.1016/j.pharep.2014.06.011
DO - 10.1016/j.pharep.2014.06.011
M3 - Artículo
C2 - 25443722
SN - 2299-5684
VL - 66
SP - 964
EP - 971
JO - Pharmacological Reports
JF - Pharmacological Reports
IS - 6
ER -