TY - JOUR
T1 - Role of the striatum in the humoral immune response to thymus-independent and thymus-dependent antigens in rats
AU - Rivera-Aguilar, Victor
AU - Querejeta, Enrique
AU - Jarillo-Luna, Rosa Adriana
AU - Reyna-Garfias, Humberto
AU - Ponce-Franco, Daniel
AU - Milliar-Garcia, Angel
AU - Quiñones-Cárdenas, Alma Rosa
AU - Pacheco-Yepez, Judith
AU - Campos-Rodríguez, Rafael
N1 - Funding Information:
Thanks to Allan Larsen for the skillful technical assistance. This work was supported by SIP-IPN.
PY - 2008/9/30
Y1 - 2008/9/30
N2 - Since the role of striatal GABAergic medium-sized spiny (MSP) neurons in the modulation of the immune responses is largely unknown, we evaluated the humoral immune response in rats with bilateral lesion of the striatum caused by quinolinic acid, which destroys MSP neurons. Sham-operated rats and those with striatal lesions were immunized either with TNP-LPS, a T-independent antigen type 1, or one of several T-dependent antigens: ovoalbumin, bovine serum albumin, lysozyme, sheep red blood cells (SRBC) or outer membrane proteins (OMP) of Salmonella enterica serovar Typhimurium. The specific levels of serum IgM and IgG, as well as intestinal IgA antibodies were determined either by enzyme-linked immunosorbent assay (ELISA) or a haemagglutination assay 5 or 7 days after immunization. Our results show that the lesion of striatal MSP neurons attenuated the primary antibody response to the T-independent antigen type 1 (TNP-LPS), but increased the antibody response to T-dependent antigens (proteins, SRBC and OMP), indicating that the striatal neurons modulate the humoral immune response in rats. The mechanisms involved are probably related to a reduction in both the number of B cells and the expression of caveolin-1 in the spleen, as well as an increase in the number of CD4+ T cells and in corticosterone levels of the serum.
AB - Since the role of striatal GABAergic medium-sized spiny (MSP) neurons in the modulation of the immune responses is largely unknown, we evaluated the humoral immune response in rats with bilateral lesion of the striatum caused by quinolinic acid, which destroys MSP neurons. Sham-operated rats and those with striatal lesions were immunized either with TNP-LPS, a T-independent antigen type 1, or one of several T-dependent antigens: ovoalbumin, bovine serum albumin, lysozyme, sheep red blood cells (SRBC) or outer membrane proteins (OMP) of Salmonella enterica serovar Typhimurium. The specific levels of serum IgM and IgG, as well as intestinal IgA antibodies were determined either by enzyme-linked immunosorbent assay (ELISA) or a haemagglutination assay 5 or 7 days after immunization. Our results show that the lesion of striatal MSP neurons attenuated the primary antibody response to the T-independent antigen type 1 (TNP-LPS), but increased the antibody response to T-dependent antigens (proteins, SRBC and OMP), indicating that the striatal neurons modulate the humoral immune response in rats. The mechanisms involved are probably related to a reduction in both the number of B cells and the expression of caveolin-1 in the spleen, as well as an increase in the number of CD4+ T cells and in corticosterone levels of the serum.
KW - CD4 T cells
KW - Caveolin-1
KW - Corticosterone
KW - GABAergic neurons
KW - Humoral immune response
KW - Striatum
KW - T-dependent antigen
KW - T-independent antigens
UR - http://www.scopus.com/inward/record.url?scp=50849143151&partnerID=8YFLogxK
U2 - 10.1016/j.imlet.2008.06.006
DO - 10.1016/j.imlet.2008.06.006
M3 - Artículo
C2 - 18639583
SN - 0165-2478
VL - 120
SP - 20
EP - 28
JO - Immunology Letters
JF - Immunology Letters
IS - 1-2
ER -