TY - JOUR
T1 - Role of the spinal Na +/H + exchanger in formalin-induced nociception
AU - Castañeda-Corral, Gabriela
AU - Rocha-González, Héctor I.
AU - Godínez-Chaparro, Beatriz
AU - Jiménez-Andrade, Juan Miguel
AU - Granados-Soto, Vinicio
N1 - Funding Information:
Gabriela Castañeda-Corral and Beatriz Godínez-Chaparro are Conacyt fellows. During the stay at the University of Arizona Gabriela Castañeda-Corral received the Beca mixta from Conacyt. This work is part of the Ph.D. dissertation of Gabriela Castañeda-Corral. Partially supported by Conacyt , grant 59879 (VG-S) .
PY - 2011/8/21
Y1 - 2011/8/21
N2 - This study assessed the role of the Na +/H + exchanger (NHE) in the formalin-induced nociception as well as the expression of the NHE isoform 1 (NHE1) in the rat spinal cord by using immunohistochemistry. Rats received a 50μl injection of diluted formalin (0.5%). Nociceptive behavior was quantified as the number of flinches of the injected paw. Intrathecal administration of the partially selective NHE1 inhibitors DMA, EIPA (0.3-30μM/rat) and the selective NHE1 inhibitor zoniporide (0.03-3μM/rat) significantly increased formalin-induced flinching behavior in a dose-dependent manner during both phases of the test. Immunohistochemical analysis of the rat lumbar spinal cord showed that NHE1 was mainly expressed in the lamina I of the dorsal horn of the spinal cord. Double immunofluorescence staining showed co-localization of NHE1 with the peptide-rich sensory nerve fiber markers, substance P and calcitonin gene-related peptide, but not with markers of neuronal cell bodies (NeuN), microglia (OX-42) or astroglia (GFAP). Collectively, these pharmacological and anatomical results suggest that spinal NHE1 plays a role in formalin-induced nociception acting as a protective protein extruding H +.
AB - This study assessed the role of the Na +/H + exchanger (NHE) in the formalin-induced nociception as well as the expression of the NHE isoform 1 (NHE1) in the rat spinal cord by using immunohistochemistry. Rats received a 50μl injection of diluted formalin (0.5%). Nociceptive behavior was quantified as the number of flinches of the injected paw. Intrathecal administration of the partially selective NHE1 inhibitors DMA, EIPA (0.3-30μM/rat) and the selective NHE1 inhibitor zoniporide (0.03-3μM/rat) significantly increased formalin-induced flinching behavior in a dose-dependent manner during both phases of the test. Immunohistochemical analysis of the rat lumbar spinal cord showed that NHE1 was mainly expressed in the lamina I of the dorsal horn of the spinal cord. Double immunofluorescence staining showed co-localization of NHE1 with the peptide-rich sensory nerve fiber markers, substance P and calcitonin gene-related peptide, but not with markers of neuronal cell bodies (NeuN), microglia (OX-42) or astroglia (GFAP). Collectively, these pharmacological and anatomical results suggest that spinal NHE1 plays a role in formalin-induced nociception acting as a protective protein extruding H +.
KW - NHE inhibitors
KW - NHE1
KW - NHE1 like-immunoreactivity
KW - Spinal processing
KW - Zoniporide
UR - http://www.scopus.com/inward/record.url?scp=79961009962&partnerID=8YFLogxK
U2 - 10.1016/j.neulet.2011.06.048
DO - 10.1016/j.neulet.2011.06.048
M3 - Comentario/Debate
C2 - 21763398
SN - 0304-3940
VL - 501
SP - 4
EP - 9
JO - Neuroscience Letters
JF - Neuroscience Letters
IS - 1
ER -