Role of the orphan receptors GPR21, GPR39, GPR82 and GPR6 in chronic inflammation in 3T3-L1 adipose model

Introduction Chronic or low-level inflammation is a process where various immune cells are recruited from the periphery into adipose tissue. This event gives rise to localized inflammations, in addition to having a close interaction with cardiometabolic pathologies where the mediation of orphan receptors is observed. Objective To analyse the participation of the orphan receptors GPR21, GPR39, GPR82 and GPR6 in a chronic inflammatory process in 3T3-L1 cells. Methods the cell culture of 3T3-L1 was made in 6 wells plate (4 x105 cell per well) in DMEM/F12 medium. The 3T3-L1 cell were stimulated with TNF-α (10mM) during 30 minutes as an inflammatory model in the cells. The gene expression was measured by RT-qPCR. Results our result showed that the inflammatory stimulus with TNF-α in adipocytes decrease the expression of the orphan receptor GPR21 GPR26, GPR39, GPR82 and GPR6 that are related with low grade inflammation. Conclusion the orphan receptors expressed in adipocytes have a main role in the TNF-α induces inflammation process, and they could be a therapeutic target to decrease the inflammation in some diseases related to low grade inflammation as diabetes, obesity and metabolic syndrome.