TY - JOUR
T1 - Isolated rhizobacteria of Jatropha curcas L.
T2 - Antagonistic activity of phytopathogens and plant growth promoter
AU - Toledo-Hernández, Erubiel
AU - Peña-Chora, Guadalupe
AU - Toribio-Jiménez, Jeiry
AU - Romero-Ramírez, Yanet
AU - Bolaños-Dircio, Alejandro
AU - Velázquez-Del Valle, Miguel G.
AU - Hernández-Lauzardo, Ana N.
AU - León-Rodríguez, Renato
AU - Vero, Silvana
N1 - Publisher Copyright:
© 2020 Universidad Autonoma Chapingo. All rights reserved.
PY - 2020/5
Y1 - 2020/5
N2 - Introduction: Jatropha curcas L. is a species native to Mexico that has recently been considered an important crop to produce biofuel. Objective: To isolate and identify rhizobacteria from J. curcas with antagonistic activity against phytopathogenic fungi (Curvularia lunata and Fusarium equiseti) of this crop and evaluate their capacity to promote tomato plant growth. Materials and methods: The isolates were obtained by serial dilution and antagonistic activity was assessed by volatile and diffusible compounds on PDA medium. In addition, the production of antifungal compounds and metabolites that promote the plant growth were determined. The isolates were identified by amplifying the 16S RNA and sodA genes. Finally, the isolates were inoculated on tomato seeds to evaluated plant growth promotion and germination. Results and discussion: The antagonistic bacteria were identified as Bacillus mojavensis, Bacillus subtilis, Bacillus thuringiensis, and Pseudomonas aeruginosa. The antifungal effect on the mycelial growth of C. lunata and F. equiseti was due to the presence of volatile and diffusible compounds with between 30 % and 79 % inhibition. Pseudomonas aeruginosa showed the strongest inhibitory effect (48.7 to 79.8 %). Bacillus subtilis (A1), B. mojavensis (A4) and B. thuringiensis (A6, A8) showed the highest germination (70 % to 88 %). The vegetative growth of tomato plants treated with the isolates was significantly higher than the controls (P < 0.05). Conclusion: Eight rhizobacteria isolated from J. curcas showed antagonistic activity against C. lunata and F. equiseti and demonstrated their capacity to improve the germination and promote growth in tomato plants.
AB - Introduction: Jatropha curcas L. is a species native to Mexico that has recently been considered an important crop to produce biofuel. Objective: To isolate and identify rhizobacteria from J. curcas with antagonistic activity against phytopathogenic fungi (Curvularia lunata and Fusarium equiseti) of this crop and evaluate their capacity to promote tomato plant growth. Materials and methods: The isolates were obtained by serial dilution and antagonistic activity was assessed by volatile and diffusible compounds on PDA medium. In addition, the production of antifungal compounds and metabolites that promote the plant growth were determined. The isolates were identified by amplifying the 16S RNA and sodA genes. Finally, the isolates were inoculated on tomato seeds to evaluated plant growth promotion and germination. Results and discussion: The antagonistic bacteria were identified as Bacillus mojavensis, Bacillus subtilis, Bacillus thuringiensis, and Pseudomonas aeruginosa. The antifungal effect on the mycelial growth of C. lunata and F. equiseti was due to the presence of volatile and diffusible compounds with between 30 % and 79 % inhibition. Pseudomonas aeruginosa showed the strongest inhibitory effect (48.7 to 79.8 %). Bacillus subtilis (A1), B. mojavensis (A4) and B. thuringiensis (A6, A8) showed the highest germination (70 % to 88 %). The vegetative growth of tomato plants treated with the isolates was significantly higher than the controls (P < 0.05). Conclusion: Eight rhizobacteria isolated from J. curcas showed antagonistic activity against C. lunata and F. equiseti and demonstrated their capacity to improve the germination and promote growth in tomato plants.
KW - Bacillus mojavensis
KW - Bacillus subtilis
KW - Bacillus thuringiensis
KW - Curvularia lunata
KW - Fusarium equiseti
KW - Pseudomonas aeruginosa
UR - http://www.scopus.com/inward/record.url?scp=85106528064&partnerID=8YFLogxK
U2 - 10.5154/R.RCHSCFA.2020.04.029
DO - 10.5154/R.RCHSCFA.2020.04.029
M3 - Artículo
AN - SCOPUS:85106528064
SN - 2007-3828
VL - 27
SP - 181
EP - 198
JO - Revista Chapingo, Serie Ciencias Forestales y del Ambiente
JF - Revista Chapingo, Serie Ciencias Forestales y del Ambiente
IS - 2
ER -