TY - JOUR
T1 - Repeated restraint stress increases IgA concentration in rat small intestine
AU - Reyna-Garfias, Humberto
AU - Miliar, Angel
AU - Jarillo-Luna, Adriana
AU - Rivera-Aguilar, Víctor
AU - Pacheco-Yepez, Judith
AU - Baeza, Isabel
AU - Campos-Rodríguez, Rafael
N1 - Funding Information:
We thank Bruce Allan Larsen for reviewing the use of English in this manuscript. This study was supported by grants 33993-N ( CONACYT , Mexico) and 20050397 ( SIP-IPN , Mexico).
PY - 2010/1
Y1 - 2010/1
N2 - The most abundant intestinal immunoglobulin and first line of specific immunological defense against environmental antigens is secretory immunoglobulin A. To better understand the effect of repeated stress on the secretion of intestinal IgA, the effects of restraint stress on IgA concentration and mRNA expression of the gene for the alpha-chain of IgA was assessed in both the duodenum and ileum of the rats. Restraint stress induced an increase in intestinal IgA, which was blocked by an adrenalectomy, suggesting a role of catecholamines and glucocorticoids. Whereas the blocking of glucocorticoid receptors by RU-486 did not affect the increased IgA concentration, it did reduce IgA alpha-chain mRNA expression in both segments, indicating a possible mediation on the part of glucocorticoids in IgA secretion by individual cells. Treatment with corticosterone significantly increased both the IgA concentration and IgA alpha-chain mRNA expression in ileum but not in duodenum, suggesting that glucocorticoids may act directly on IgA-antibody forming cells to increase IgA secretion in the former segment. A probable role by catecholamines was evidenced by the reduction in IgA concentration and IgA alpha-chain mRNA expression in both segments after a chemical sympathectomy with 6-hydroxydopamine (6-OHDA). Additionally, norepinephrine significantly reduced IgA alpha-chain mRNA levels but increased pIgR mRNA expression and IgA concentration in both intestinal segments. We propose that the increased intestinal IgA levels caused by repeated restraint stress is likely due to the effects of catecholamines on the transport of plgA across the epithelium.
AB - The most abundant intestinal immunoglobulin and first line of specific immunological defense against environmental antigens is secretory immunoglobulin A. To better understand the effect of repeated stress on the secretion of intestinal IgA, the effects of restraint stress on IgA concentration and mRNA expression of the gene for the alpha-chain of IgA was assessed in both the duodenum and ileum of the rats. Restraint stress induced an increase in intestinal IgA, which was blocked by an adrenalectomy, suggesting a role of catecholamines and glucocorticoids. Whereas the blocking of glucocorticoid receptors by RU-486 did not affect the increased IgA concentration, it did reduce IgA alpha-chain mRNA expression in both segments, indicating a possible mediation on the part of glucocorticoids in IgA secretion by individual cells. Treatment with corticosterone significantly increased both the IgA concentration and IgA alpha-chain mRNA expression in ileum but not in duodenum, suggesting that glucocorticoids may act directly on IgA-antibody forming cells to increase IgA secretion in the former segment. A probable role by catecholamines was evidenced by the reduction in IgA concentration and IgA alpha-chain mRNA expression in both segments after a chemical sympathectomy with 6-hydroxydopamine (6-OHDA). Additionally, norepinephrine significantly reduced IgA alpha-chain mRNA levels but increased pIgR mRNA expression and IgA concentration in both intestinal segments. We propose that the increased intestinal IgA levels caused by repeated restraint stress is likely due to the effects of catecholamines on the transport of plgA across the epithelium.
KW - Catecholamines
KW - Glucocorticoids
KW - IgA
KW - Polymeric Ig receptor
KW - Restraint-stress
KW - Small intestine
UR - http://www.scopus.com/inward/record.url?scp=71749101551&partnerID=8YFLogxK
U2 - 10.1016/j.bbi.2009.09.005
DO - 10.1016/j.bbi.2009.09.005
M3 - Artículo
C2 - 19748568
SN - 0889-1591
VL - 24
SP - 110
EP - 118
JO - Brain, Behavior, and Immunity
JF - Brain, Behavior, and Immunity
IS - 1
ER -