TY - JOUR
T1 - Regulation of two photosynthetic pigment-related genes during stress-induced pigment formation in the green alga, Dunaliella salina
AU - Sánchez-Estudillo, Leticia
AU - Freile-Pelegrin, Yolanda
AU - Rivera-Madrid, Renata
AU - Robledo, Daniel
AU - Narváez-Zapata, José Alberto
N1 - Funding Information:
Acknowledgments This research was financed by the CONACyT (J46435-Z) and SAGARPA (2002-C01-1057). The authors thank Margarita Aguilar Espinosa for technical assistance.
PY - 2006/6
Y1 - 2006/6
N2 - The expression of mRNAs coding for 1-deoxyxylulose-5-phosphate synthase (DXS) and phytoene synthase (PSY) were studied in Dunaliella salina grown under nitrogen-sufficient (NS) and nitrogen-limited (NL) conditions. Under NS conditions growth was 2.5 times higher than under NL conditions. No differences were found in chlorophyll a content per cell, and total carotenoid content per cell was 5.33 pg 1-1 for the NS treatment and 7.76 pg 1-1 for the NL. DXS transcripts exhibited diminished expression under NL conditions, peaking at day 15 of cultivation in both treatments. Simultaneously, PSY transcripts exhibited constant expression under both conditions. These results suggest that these genes play an important role in the balance of photosynthetic pigments during pigment accumulation.
AB - The expression of mRNAs coding for 1-deoxyxylulose-5-phosphate synthase (DXS) and phytoene synthase (PSY) were studied in Dunaliella salina grown under nitrogen-sufficient (NS) and nitrogen-limited (NL) conditions. Under NS conditions growth was 2.5 times higher than under NL conditions. No differences were found in chlorophyll a content per cell, and total carotenoid content per cell was 5.33 pg 1-1 for the NS treatment and 7.76 pg 1-1 for the NL. DXS transcripts exhibited diminished expression under NL conditions, peaking at day 15 of cultivation in both treatments. Simultaneously, PSY transcripts exhibited constant expression under both conditions. These results suggest that these genes play an important role in the balance of photosynthetic pigments during pigment accumulation.
KW - Carotenoids
KW - Dunaliella salina
KW - Gene expression
UR - http://www.scopus.com/inward/record.url?scp=33745256751&partnerID=8YFLogxK
U2 - 10.1007/s10529-006-9001-2
DO - 10.1007/s10529-006-9001-2
M3 - Artículo
C2 - 16786242
SN - 0141-5492
VL - 28
SP - 787
EP - 791
JO - Biotechnology Letters
JF - Biotechnology Letters
IS - 11
ER -