TY - JOUR
T1 - Regulation of macrophage gene expression by Mycobacterium tuberculosis
T2 - Down-regulation of mitochondrial cytochrome C oxidase
AU - Ragno, Silvia
AU - Estrada-Garcia, Iris
AU - Butler, Robert
AU - Colston, M. Joseph
PY - 1998/8
Y1 - 1998/8
N2 - We have investigated changes in gene expression in mouse peritoneal macrophages following infection with virulent Mycobacterium tuberculosis. Using differential-display reverse transcription-PCR (RT-PCR), we have identified a gene that was markedly down-regulated within 6 h of infection and remained so for the duration of the experiment (5 days). On sequencing, this gene was found to encode the murine cytochrome c oxidase subunit VIIc (COX VIIc). Down-regulation of COX VIIc during M. tuberculosis infection was confirmed by three independent techniques: limiting-dilution RT-PCR, RNase protection assay, and Northern analysis. Limiting-dilution RT-PCR and Northern analysis were also used to analyze the specificity of this regulation; heat-killed M. tuberculosis, Mycobacterium bovis BCG, and latex beads had no effect on expression of COX VIIc. Downregulation of this enzyme was also confirmed by using adherent cells isolated from spleens of M. tuberculosis infected mice. These ex vivo macrophages showed apoptotic features, suggesting a possible involvement of cytochrome c oxidase in the programmed cell death of the host cells.
AB - We have investigated changes in gene expression in mouse peritoneal macrophages following infection with virulent Mycobacterium tuberculosis. Using differential-display reverse transcription-PCR (RT-PCR), we have identified a gene that was markedly down-regulated within 6 h of infection and remained so for the duration of the experiment (5 days). On sequencing, this gene was found to encode the murine cytochrome c oxidase subunit VIIc (COX VIIc). Down-regulation of COX VIIc during M. tuberculosis infection was confirmed by three independent techniques: limiting-dilution RT-PCR, RNase protection assay, and Northern analysis. Limiting-dilution RT-PCR and Northern analysis were also used to analyze the specificity of this regulation; heat-killed M. tuberculosis, Mycobacterium bovis BCG, and latex beads had no effect on expression of COX VIIc. Downregulation of this enzyme was also confirmed by using adherent cells isolated from spleens of M. tuberculosis infected mice. These ex vivo macrophages showed apoptotic features, suggesting a possible involvement of cytochrome c oxidase in the programmed cell death of the host cells.
UR - http://www.scopus.com/inward/record.url?scp=0031848309&partnerID=8YFLogxK
U2 - 10.1128/iai.66.8.3952-3958.1998
DO - 10.1128/iai.66.8.3952-3958.1998
M3 - Artículo
SN - 0019-9567
VL - 66
SP - 3952
EP - 3958
JO - Infection and Immunity
JF - Infection and Immunity
IS - 8
ER -