TY - JOUR
T1 - Quantification of vitellin/vitellogenin-like proteins in the oyster Crassostrea corteziensis (Hertlein 1951) as a tool to predict the degree of gonad maturity
AU - Arcos, Fabiola G.
AU - Ibarra, Ana María
AU - Rodríguez-Jaramillo, María Del Carmen
AU - García-Latorre, Ethel Awilda
AU - Vazquez-Boucard, Celia
PY - 2009/4
Y1 - 2009/4
N2 - The oyster's reproductive process is poorly documented, especially in terms of a quantitative approach. In recent years, investigations with this species have been directed at determining important reproductive factors. Within this scope, techniques that allow standardized and accurate quantitative estimations of gonad development have become of primary importance. In this study, the histological characteristics and the levels of vitellin/vitellogenin-like proteins (Vn/Vtg) from ovaries of the Mexican Pacific 'pleasure' oyster Crassostrea corteziensis (Hertlein 1951) were analysed during different stages of gonad maturation using quantitative histological techniques and an enzyme-linked immunosorbent assay. This was performed in order to evaluate a possible quantitative tool to predict the degrees of gonad maturity and to analyse the biological implications of the findings relative not only to broodstock conditioning but also to natural populations. Using this information, we expect to be able to undertake further research on different reproductive aspects of this oyster species, including, among others, evaluation of the response in Vn/Vtg concentrations to different diets and environmental conditions during laboratory conditioning.
AB - The oyster's reproductive process is poorly documented, especially in terms of a quantitative approach. In recent years, investigations with this species have been directed at determining important reproductive factors. Within this scope, techniques that allow standardized and accurate quantitative estimations of gonad development have become of primary importance. In this study, the histological characteristics and the levels of vitellin/vitellogenin-like proteins (Vn/Vtg) from ovaries of the Mexican Pacific 'pleasure' oyster Crassostrea corteziensis (Hertlein 1951) were analysed during different stages of gonad maturation using quantitative histological techniques and an enzyme-linked immunosorbent assay. This was performed in order to evaluate a possible quantitative tool to predict the degrees of gonad maturity and to analyse the biological implications of the findings relative not only to broodstock conditioning but also to natural populations. Using this information, we expect to be able to undertake further research on different reproductive aspects of this oyster species, including, among others, evaluation of the response in Vn/Vtg concentrations to different diets and environmental conditions during laboratory conditioning.
KW - Crassostrea corteziensis
KW - ELISA assay
KW - Gonad development
KW - Histology
KW - Oyster
KW - Vitellin/vitellogenin-like protein
UR - http://www.scopus.com/inward/record.url?scp=63849099789&partnerID=8YFLogxK
U2 - 10.1111/j.1365-2109.2008.02138.x
DO - 10.1111/j.1365-2109.2008.02138.x
M3 - Artículo
AN - SCOPUS:63849099789
SN - 1355-557X
VL - 40
SP - 644
EP - 655
JO - Aquaculture Research
JF - Aquaculture Research
IS - 6
ER -