TY - JOUR
T1 - Purification, crystallization and preliminary X-ray analysis of Mexicain
AU - Oliver-Salvador, M. C.
AU - González-Ramírez, L. A.
AU - Gavira, J. A.
AU - Soriano-García, M.
AU - García-Ruiz, J. M.
PY - 2004/11
Y1 - 2004/11
N2 - Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 Å and belong to the monoclinic space group P21, with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 Å, β = 92.64°. The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (VM) of 2.24 Å3 Da-1 and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.
AB - Mexicain is a 23.7 kDa papain-like cysteine protease from the tropical plant Jacaratia mexicana. Extracted as a mix of proteases from the latex of the fruit, mexicain is isolated after cation-exchange chromatography as the most abundant product. The purified product inhibited with E-64 was crystallized by sitting-drop vapour diffusion in the presence of ethanolamine. Cryoprotected crystals diffracted X-rays from a home source to 1.98 Å and belong to the monoclinic space group P21, with unit-cell parameters a = 57.36, b = 90.45, c = 80.39 Å, β = 92.64°. The asymmetric unit contains four molecules of mexicain, with a corresponding crystal volume per protein weight (VM) of 2.24 Å3 Da-1 and a solvent content of 45% by volume. A molecular-replacement model has been determined and refinement is in progress.
UR - http://www.scopus.com/inward/record.url?scp=21644441049&partnerID=8YFLogxK
U2 - 10.1107/S0907444904021638
DO - 10.1107/S0907444904021638
M3 - Artículo
SN - 0907-4449
VL - 60
SP - 2058
EP - 2060
JO - Acta Crystallographica Section D: Biological Crystallography
JF - Acta Crystallographica Section D: Biological Crystallography
IS - 11
ER -