TY - JOUR
T1 - Proteomic comparison of biofilm vs. planktonic Staphylococcus epidermidis cells suggests key metabolic differences between these conditions
AU - Martínez-García, Sergio
AU - Peralta, Humberto
AU - Betanzos-Cabrera, Gabriel
AU - Chavez-Galan, Leslie
AU - Rodríguez-Martínez, Sandra
AU - Cancino-Diaz, Mario E.
AU - Cancino-Diaz, Juan C.
N1 - Publisher Copyright:
© 2021 Institut Pasteur
PY - 2021/3
Y1 - 2021/3
N2 - Previous studies have shown that biofilm-forming bacteria are deficient in tricarboxylic acid (TCA) cycle metabolites, suggesting a relationship between these cellular processes. In this work, we compared the proteomes of planktonic vs biofilm cells from a clinical strain of Staphylococcus epidermidis using LC-MS/MS. A total of 168 proteins were identified from both growth conditions. The biofilm cells showed enrichment of proteins participating in glycolysis for the formation of pyruvate; however, the absence of TCA cycle proteins and the presence of lactate dehydrogenase, formate acetyltransferase, and acetoin reductase suggested that pyruvate was catabolized to their respective products: lactate, formate and acetoin. On the other hand, planktonic cells showed proteins participating in glycolysis and the TCA cycle, the pentose phosphate pathway, gluconeogenesis, ATP generation and the oxidative stress response. Functional networks with higher interconnection were predicted for planktonic proteins. We propose that in S. epidermidis, the relative absence of TCA cycle proteins is associated with the formation of biofilms and that lactate, formate and acetoin are the end products of partial glucose metabolism.
AB - Previous studies have shown that biofilm-forming bacteria are deficient in tricarboxylic acid (TCA) cycle metabolites, suggesting a relationship between these cellular processes. In this work, we compared the proteomes of planktonic vs biofilm cells from a clinical strain of Staphylococcus epidermidis using LC-MS/MS. A total of 168 proteins were identified from both growth conditions. The biofilm cells showed enrichment of proteins participating in glycolysis for the formation of pyruvate; however, the absence of TCA cycle proteins and the presence of lactate dehydrogenase, formate acetyltransferase, and acetoin reductase suggested that pyruvate was catabolized to their respective products: lactate, formate and acetoin. On the other hand, planktonic cells showed proteins participating in glycolysis and the TCA cycle, the pentose phosphate pathway, gluconeogenesis, ATP generation and the oxidative stress response. Functional networks with higher interconnection were predicted for planktonic proteins. We propose that in S. epidermidis, the relative absence of TCA cycle proteins is associated with the formation of biofilms and that lactate, formate and acetoin are the end products of partial glucose metabolism.
KW - Biofilm
KW - Formate
KW - Planktonic
KW - Proteomic
KW - Staphylococcus epidermidis
KW - Tricarboxylic acids
UR - http://www.scopus.com/inward/record.url?scp=85102641551&partnerID=8YFLogxK
U2 - 10.1016/j.resmic.2020.103796
DO - 10.1016/j.resmic.2020.103796
M3 - Artículo
C2 - 33412274
AN - SCOPUS:85102641551
SN - 0923-2508
VL - 172
JO - Research in Microbiology
JF - Research in Microbiology
IS - 2
M1 - 103796
ER -