TY - JOUR
T1 - Protective Effect of Chickpea Protein Hydrolysates on Colon Carcinogenesis Associated With a Hypercaloric Diet
AU - Sánchez-Chino, Xariss M.
AU - Jiménez Martínez, Cristian
AU - León-Espinosa, Erika B.
AU - Garduño-Siciliano, Leticia
AU - Álvarez-González, Isela
AU - Madrigal-Bujaidar, Eduardo
AU - Vásquez-Garzón, Verónica R.
AU - Baltiérrez-Hoyos, Rafael
AU - Dávila-Ortiz, Gloria
N1 - Publisher Copyright:
© 2018, © 2018 American College of Nutrition.
PY - 2019/2/17
Y1 - 2019/2/17
N2 - Objective: Colon cancer occupies the third place in incidence worldwide; eating habits, in particular, consumption of hypercaloric diets, are relevant in its etiopathogenesis. On the other hand, foods can also modulate carcinogenesis: for example, proteins, which when hydrolyzed release peptides with biological activities, and legumes, especially, chickpea, represent a good source of hydrolysates. The objective of this work was to verify the inhibitory effect of chickpea hydrolyzed protein on azoxymethane (AOM)-induced carcinogenesis in mice fed a hypercaloric diet. Methods: We hydrolyzed chickpea protein by pepsin, pancreatin, and a combined pepsin–pancreatin system, to test its anticarcinogenic and hypercaloric activity in mice that had consumed a hypercaloric diet or a normal diet but were injected with azoxymethane (AOM). Results: A concentrate (70% proteins) was obtained from chickpea seeds (18.5% proteins), and extensive hydrolysates were obtained at 15 minutes, in all tested enzyme systems. The greatest activity was evidenced in the hydrolysates obtained with pepsin–pancreatin at 90 minutes. Animals that consumed the hypercaloric diet had a higher concentration of cholesterol and a higher atherogenic index, which were significantly reduced with the administration of chickpea protein hydrolysates with a dose-response effect (10, 20, or 30 mg/kg), whereas no effect was observed in animals that consumed the normal diet. In animals given AOM, aberrant crypts were observed, at a higher rate in animals that consumed the hypercaloric diet; with the consumption of hydrolysates by the animals that consumed either diet, the number of aberrant crypts was reduced with the 3 doses tested, and the effect was better in those animals fed the hypercaloric diet. The best effect in all tests was with 30 mg/kg body weight. Conclusion: The consumption of chickpea protein hydrolysates might confer a protective effect against colon carcinogenesis.
AB - Objective: Colon cancer occupies the third place in incidence worldwide; eating habits, in particular, consumption of hypercaloric diets, are relevant in its etiopathogenesis. On the other hand, foods can also modulate carcinogenesis: for example, proteins, which when hydrolyzed release peptides with biological activities, and legumes, especially, chickpea, represent a good source of hydrolysates. The objective of this work was to verify the inhibitory effect of chickpea hydrolyzed protein on azoxymethane (AOM)-induced carcinogenesis in mice fed a hypercaloric diet. Methods: We hydrolyzed chickpea protein by pepsin, pancreatin, and a combined pepsin–pancreatin system, to test its anticarcinogenic and hypercaloric activity in mice that had consumed a hypercaloric diet or a normal diet but were injected with azoxymethane (AOM). Results: A concentrate (70% proteins) was obtained from chickpea seeds (18.5% proteins), and extensive hydrolysates were obtained at 15 minutes, in all tested enzyme systems. The greatest activity was evidenced in the hydrolysates obtained with pepsin–pancreatin at 90 minutes. Animals that consumed the hypercaloric diet had a higher concentration of cholesterol and a higher atherogenic index, which were significantly reduced with the administration of chickpea protein hydrolysates with a dose-response effect (10, 20, or 30 mg/kg), whereas no effect was observed in animals that consumed the normal diet. In animals given AOM, aberrant crypts were observed, at a higher rate in animals that consumed the hypercaloric diet; with the consumption of hydrolysates by the animals that consumed either diet, the number of aberrant crypts was reduced with the 3 doses tested, and the effect was better in those animals fed the hypercaloric diet. The best effect in all tests was with 30 mg/kg body weight. Conclusion: The consumption of chickpea protein hydrolysates might confer a protective effect against colon carcinogenesis.
KW - Legumes
KW - aberrant crypts
KW - colon cancer
KW - hyperlipidemia
KW - protein hydrolyzates
UR - http://www.scopus.com/inward/record.url?scp=85053459600&partnerID=8YFLogxK
U2 - 10.1080/07315724.2018.1487809
DO - 10.1080/07315724.2018.1487809
M3 - Artículo
C2 - 30211662
SN - 0731-5724
VL - 38
SP - 162
EP - 170
JO - Journal of the American College of Nutrition
JF - Journal of the American College of Nutrition
IS - 2
ER -