TY - JOUR
T1 - Prognostic Value of CD95, Active Caspase-3, and Bcl-2 Expression in Adult Patients with De Novo Acute Lymphoblastic Leukemia
AU - Montiel-Cervantes, Laura Arcelia
AU - Reyes-Maldonado, Elba
AU - Garcia-Chavez, Jaime
AU - Hernandez-Caballero, Alvaro
AU - Molina-Aguilar, Rubiraida
AU - Garcia-Ruiz Esparza, Miriam America
AU - Vela-Ojeda, Jorge
N1 - Publisher Copyright:
© 2018 IMSS
PY - 2018/1
Y1 - 2018/1
N2 - Background: Acute lymphoblastic leukemia is an aggressive malignant disease with high mortality rates in adults. Aim of the study: The expression levels of CD95, active caspase-3, and Bcl-2 were determined in 111 patients with de novo acute lymphoblastic leukemia (ALL) and correlated with overall survival (OS) and disease-free survival (DFS). Methods: The immunophenotyped ok leukemia and the expression of CD95, active caspase-3, and Bcl-2, were determined by flow cytometry. Apoptotic variables were correlated by Spearman test, and survival by Kaplan-Meier method. Log-rank test was used to compare survival curves. Results: From a total of 111 patients, 56 cases were B-ALL, 16 T-ALL, 16 B-ALL/CD33+, and 23 ambiguous lineage-AL (AmbLin-AL). The median expression of CD95 (61.5%) and active-caspase-3 (19.4%) was higher in T-ALL (p <0.05), whereas Bcl-2 was lower in T-ALL (p <0.038). There was a highly significant correlation in B-ALL, B-ALL/CD33+ and AmbLin-AL between CD95 and Bcl-2, CD95-Active caspase-3, and Bcl-2-Active caspase-3; while in T-ALL, there was only a correlation between CD95-Active caspase-3, and Bcl-2-Active caspase-3. OS and DFS were better for T-ALL than the other groups, especially in patients having higher values of CD95 and active caspase 3, and lower values of Bcl-2. The worse survival rates were observed in patients with B-ALL/CD33+, and AmbLin-AL. Conclusions: The prognosis of ALL in adults is influenced by the expression levels of Bcl-2, active-caspase-3, and CD95.
AB - Background: Acute lymphoblastic leukemia is an aggressive malignant disease with high mortality rates in adults. Aim of the study: The expression levels of CD95, active caspase-3, and Bcl-2 were determined in 111 patients with de novo acute lymphoblastic leukemia (ALL) and correlated with overall survival (OS) and disease-free survival (DFS). Methods: The immunophenotyped ok leukemia and the expression of CD95, active caspase-3, and Bcl-2, were determined by flow cytometry. Apoptotic variables were correlated by Spearman test, and survival by Kaplan-Meier method. Log-rank test was used to compare survival curves. Results: From a total of 111 patients, 56 cases were B-ALL, 16 T-ALL, 16 B-ALL/CD33+, and 23 ambiguous lineage-AL (AmbLin-AL). The median expression of CD95 (61.5%) and active-caspase-3 (19.4%) was higher in T-ALL (p <0.05), whereas Bcl-2 was lower in T-ALL (p <0.038). There was a highly significant correlation in B-ALL, B-ALL/CD33+ and AmbLin-AL between CD95 and Bcl-2, CD95-Active caspase-3, and Bcl-2-Active caspase-3; while in T-ALL, there was only a correlation between CD95-Active caspase-3, and Bcl-2-Active caspase-3. OS and DFS were better for T-ALL than the other groups, especially in patients having higher values of CD95 and active caspase 3, and lower values of Bcl-2. The worse survival rates were observed in patients with B-ALL/CD33+, and AmbLin-AL. Conclusions: The prognosis of ALL in adults is influenced by the expression levels of Bcl-2, active-caspase-3, and CD95.
KW - Active caspase-3
KW - Acute lymphoblastic leukemia
KW - Bcl-2
KW - CD95 (Fas)
KW - Survival
UR - http://www.scopus.com/inward/record.url?scp=85045550608&partnerID=8YFLogxK
U2 - 10.1016/j.arcmed.2018.04.006
DO - 10.1016/j.arcmed.2018.04.006
M3 - Artículo
C2 - 29680307
SN - 0188-4409
VL - 49
SP - 44
EP - 50
JO - Archives of Medical Research
JF - Archives of Medical Research
IS - 1
ER -