TY - CHAP
T1 - Production of recombinant proteins in the chloroplast of the green alga Chlamydomonas reinhardtii
AU - Guzmán-Zapata, Daniel
AU - Macedo-Osorio, Karla Soledad
AU - Almaraz-Delgado, Alma Lorena
AU - Durán-Figueroa, Noé
AU - Badillo-Corona, Jesus Agustín
N1 - Publisher Copyright:
© Springer Science+Business Media New York 2016.
PY - 2016/5/1
Y1 - 2016/5/1
N2 - Chloroplast transformation in the green algae Chlamydomonas reinhardtii can be used for the production of valuable recombinant proteins. Here, we describe chloroplast transformation of C. reinhardtii followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant proteins can be detected by conventional techniques like immunoblotting and purified from liquid cultures.
AB - Chloroplast transformation in the green algae Chlamydomonas reinhardtii can be used for the production of valuable recombinant proteins. Here, we describe chloroplast transformation of C. reinhardtii followed by protein detection. Genes of interest integrate stably by homologous recombination into the chloroplast genome following introduction by particle bombardment. Genes are inherited and expressed in lines recovered after selection in the presence of an antibiotic. Recombinant proteins can be detected by conventional techniques like immunoblotting and purified from liquid cultures.
KW - Chlamydomonas reinhardtii
KW - Chloroplast biotechnology
KW - Chloroplast transformation
KW - Green algae
KW - Particle bombardment
KW - Recombinant protein
UR - http://www.scopus.com/inward/record.url?scp=84965099342&partnerID=8YFLogxK
U2 - 10.1007/978-1-4939-3289-4_5
DO - 10.1007/978-1-4939-3289-4_5
M3 - Capítulo
C2 - 26614282
AN - SCOPUS:84965099342
T3 - Methods in Molecular Biology
SP - 69
EP - 85
BT - Methods in Molecular Biology
PB - Humana Press Inc.
ER -