TY - JOUR
T1 - Prevalence of Helicobacter pylori from the oral cavity of Mexican asymptomatic children under 5 years of age through PCR
AU - Castro-Muñoz, Leonardo Josué
AU - González-Díaz, César Antonio
AU - Muñoz-Escobar, Aide
AU - Tovar-Ayona, Brisna Joana
AU - Aguilar-Anguiano, Luz María
AU - Vargas-Olmos, Rebeca
AU - Sánchez-Monroy, Virginia
N1 - Publisher Copyright:
© 2016 Elsevier Ltd
PY - 2017/1/1
Y1 - 2017/1/1
N2 - Objetive H. pylori infection is acquired early in life, but symptoms occur mainly in adults. The mode of transmission remains unclear, but several studies have demonstrated that the microorganism may be transmitted orally. H. pylori has been detected in the oral cavity of children, and some authors have studied infection prevalence due to the importance of the bacteria in gastric and extragastric pathology. In México, little is known about H. pylori prevalence in the oral cavity of asymptomatic children, so the aims of this study were to estimate the prevalence of H. pylori in asymptomatic children and evaluate factors associated with the infection. Desing We studied 162 asymptomatic children of 3.025 ± 1.474 years of age from a kindergarten in México City. Oral swabs, rotating around to cheeks inside of oral cavity were obtained to determine H. pylori, using the polymerase chain reaction for 16S rRNA and glmM genes. Results We detected a 13% prevalence of H. pylori infection by PCR, and the results were confirmed by sequencing and showed a >97% identity with H. pylori. We observed increased infection with age. No association between H. pylori infection and variables such as sex, family history of gastric diseases, crowding or pets in the home were found. Conclusion H. pylori infection in the oral cavity is frequent among asymptomatic children in México, which represents a risk of infection for other family members and for other children in educational facilities. Early diagnosis is essential for control of the infection and for the development of preventive strategies in childhood.
AB - Objetive H. pylori infection is acquired early in life, but symptoms occur mainly in adults. The mode of transmission remains unclear, but several studies have demonstrated that the microorganism may be transmitted orally. H. pylori has been detected in the oral cavity of children, and some authors have studied infection prevalence due to the importance of the bacteria in gastric and extragastric pathology. In México, little is known about H. pylori prevalence in the oral cavity of asymptomatic children, so the aims of this study were to estimate the prevalence of H. pylori in asymptomatic children and evaluate factors associated with the infection. Desing We studied 162 asymptomatic children of 3.025 ± 1.474 years of age from a kindergarten in México City. Oral swabs, rotating around to cheeks inside of oral cavity were obtained to determine H. pylori, using the polymerase chain reaction for 16S rRNA and glmM genes. Results We detected a 13% prevalence of H. pylori infection by PCR, and the results were confirmed by sequencing and showed a >97% identity with H. pylori. We observed increased infection with age. No association between H. pylori infection and variables such as sex, family history of gastric diseases, crowding or pets in the home were found. Conclusion H. pylori infection in the oral cavity is frequent among asymptomatic children in México, which represents a risk of infection for other family members and for other children in educational facilities. Early diagnosis is essential for control of the infection and for the development of preventive strategies in childhood.
KW - Asymptomatic
KW - Cavity oral
KW - Children
KW - H. pylori
KW - PCR
KW - Prevalence
UR - http://www.scopus.com/inward/record.url?scp=84988461456&partnerID=8YFLogxK
U2 - 10.1016/j.archoralbio.2016.09.007
DO - 10.1016/j.archoralbio.2016.09.007
M3 - Artículo
C2 - 27665274
SN - 0003-9969
VL - 73
SP - 55
EP - 59
JO - Archives of Oral Biology
JF - Archives of Oral Biology
ER -