Presencia de toxinas tipo benzoato en una cepa de gymnodinium catenatum (dinophyceae) aislada de manzanillo, colima, méxico

The extract of a Gymnodinium catenatum strain, isolated from Manzanillo, Colima, in the Mexican Pacific coast, was analized by nuclear magnetic resonance (1H-NMR) to determine the presence of benzoate-type analogs. The extract was fractioned using solid-phase extraction (SPE) partitioning on a C-18 cartridge, using a stepwise gradient from 0 to 100% (v/v). Fraction 2 (10% methanol) showed two aromatic doublet signals at 7.91 and 7.53 ppm, consistent with an AA'BB' system, corresponding to a para substituted bencenic ring, attributed to the hydroxybenzoate moiety on C-17 of the toxin basic structure. These findings indicate the presence of benzoate type analogs in this Mexican strain. These analogs have been poorly studied, and the report on their existence in our country is of great importance because they could represent a public health risk, but in the other hand, their existence generates the opportunity to continue the research to determine their toxicity in mammals and, eventually, their pharmacological potential.