TY - JOUR
T1 - Prevalence of bacterial contamination in platelet concentrates at the National Center of Blood Transfusion (Mexico)
AU - Ibáñez-Cervantes, G.
AU - Bello-López, J. M.
AU - Fernández-Sánchez, V.
AU - Domínguez-Mendoza, C. A.
AU - Acevedo-Alfaro, L. I.
N1 - Publisher Copyright:
© 2017 Elsevier Masson SAS
PY - 2017/6
Y1 - 2017/6
N2 - Objectives Most common bacterial sepsis associated with transfusion is caused by contaminated Platelet Concentrates (PC). The screening of PC to detect bacterial contamination is obligatory in Mexico, and it is carried out in quality control programs. In Mexico, the identification and molecular characterization of bacterial contaminants to detect contamination sources have not been implemented due to high costs; however, it is an actual current need. Material and methods One hundred PC were randomly selected and microbiologically analyzed. This sample size corresponds to 1% of the PC obtained by the National Center of Blood Transfusion (NCBT) in Mexico City according to the Official Mexican Standard NOM-253-SSA1-2012. Additionally, molecular biology tests were implemented in order to identify the possible contamination sources. Results Nine of the 100 PC analyzed (9%) showed bacterial contamination; analysis of the nucleotide sequences revealed the presence of characteristic microbiota from donor skin and soil. Diverse clonal relationship between the strains was identified in Staphylococcus epidermidis. Conclusion Detection of contaminants associated with environmental and skin flora, shows the need to implement measures in the process of disinfecting skin at the site of phlebotomy and cleaning each of the areas involved in blood collection.
AB - Objectives Most common bacterial sepsis associated with transfusion is caused by contaminated Platelet Concentrates (PC). The screening of PC to detect bacterial contamination is obligatory in Mexico, and it is carried out in quality control programs. In Mexico, the identification and molecular characterization of bacterial contaminants to detect contamination sources have not been implemented due to high costs; however, it is an actual current need. Material and methods One hundred PC were randomly selected and microbiologically analyzed. This sample size corresponds to 1% of the PC obtained by the National Center of Blood Transfusion (NCBT) in Mexico City according to the Official Mexican Standard NOM-253-SSA1-2012. Additionally, molecular biology tests were implemented in order to identify the possible contamination sources. Results Nine of the 100 PC analyzed (9%) showed bacterial contamination; analysis of the nucleotide sequences revealed the presence of characteristic microbiota from donor skin and soil. Diverse clonal relationship between the strains was identified in Staphylococcus epidermidis. Conclusion Detection of contaminants associated with environmental and skin flora, shows the need to implement measures in the process of disinfecting skin at the site of phlebotomy and cleaning each of the areas involved in blood collection.
KW - Bacteria
KW - Blood bank
KW - Contamination
KW - Platelet concentrates
KW - Quality control
UR - http://www.scopus.com/inward/record.url?scp=85017450150&partnerID=8YFLogxK
U2 - 10.1016/j.tracli.2017.03.003
DO - 10.1016/j.tracli.2017.03.003
M3 - Artículo
C2 - 28412024
SN - 1246-7820
VL - 24
SP - 56
EP - 61
JO - Transfusion Clinique et Biologique
JF - Transfusion Clinique et Biologique
IS - 2
ER -