TY - JOUR
T1 - Plasmid DNA immunization with Trypanosoma cruzi genes induces cardiac and clinical protection against Chagas disease in the canine model
AU - Rodríguez-Morales, Olivia
AU - Pérez-Leyva, M. Magdalena
AU - Ballinas-Verdugo, Martha A.
AU - Carrillo-Sánchez, Silvia C.
AU - Rosales-Encina, J. Luis
AU - Alejandre-Aguilar, Ricardo
AU - Reyes, Pedro A.
AU - Arce-Fonseca, Minerva
N1 - Funding Information:
This research was funded by the Consejo Nacional de Ciencia y Tecnología (CONACyT) Mexico, grant No. 69081 FONSEC SSA/IMSS/ISSSTE. We thank MVZ. Yukie V. Tachika from Facultad de Medicina Veterinaria y Zootecnia of Universidad Nacional Autónoma de México for her invaluable help in EKG interpretation.
PY - 2012
Y1 - 2012
N2 - The only existing preventive measure against American trypanosomosis, or Chagas disease, is the control of the transmitting insect, which has only been effective in a few South American regions. Currently, there is no vaccine available to prevent this disease. Here, we present the clinical and cardiac levels of protection induced by expression to Trypanosoma cruzi genes encoding the TcSP and TcSSP4 proteins in the canine model. Physical examination, diagnostic chagasic serology, and serial electrocardiograms were performed before and after immunization, as well as after experimental infection. We found that immunization with recombinant plasmids prevented hyperthermia in the acute phase of experimental infection and produced lymphadenomegaly as an immunological response against the parasite and additionally prevented heart rate elevation (tachycardia) in the acute and/or chronic stages of infection. Immunization with T. cruzi genes encoding the TcSP and TcSSP4 antigens diminished the quality and quantity of the electrocardiographic abnormalities, thereby avoiding progression to more severe developments such as right bundle branch block or ventricular premature complexes in a greater number of dogs.
AB - The only existing preventive measure against American trypanosomosis, or Chagas disease, is the control of the transmitting insect, which has only been effective in a few South American regions. Currently, there is no vaccine available to prevent this disease. Here, we present the clinical and cardiac levels of protection induced by expression to Trypanosoma cruzi genes encoding the TcSP and TcSSP4 proteins in the canine model. Physical examination, diagnostic chagasic serology, and serial electrocardiograms were performed before and after immunization, as well as after experimental infection. We found that immunization with recombinant plasmids prevented hyperthermia in the acute phase of experimental infection and produced lymphadenomegaly as an immunological response against the parasite and additionally prevented heart rate elevation (tachycardia) in the acute and/or chronic stages of infection. Immunization with T. cruzi genes encoding the TcSP and TcSSP4 antigens diminished the quality and quantity of the electrocardiographic abnormalities, thereby avoiding progression to more severe developments such as right bundle branch block or ventricular premature complexes in a greater number of dogs.
UR - http://www.scopus.com/inward/record.url?scp=84870036340&partnerID=8YFLogxK
U2 - 10.1186/1297-9716-43-79
DO - 10.1186/1297-9716-43-79
M3 - Artículo
C2 - 23148870
SN - 0928-4249
VL - 43
JO - Veterinary Research
JF - Veterinary Research
IS - 1
M1 - 79
ER -