TY - JOUR
T1 - Phylogenetic analysis of the archaeal community in an alkaline-saline soil of the former lake Texcoco (Mexico)
AU - Valenzuela-Encinas, César
AU - Neria-González, Isabel
AU - Alcántara-Hernández, Rocio J.
AU - Enríquez-Aragón, J. Arturo
AU - Estrada-Alvarado, Isabel
AU - Hernández-Rodríguez, César
AU - Dendooven, Luc
AU - Marsch, Rodolfo
N1 - Funding Information:
Acknowledgments This work was funded by ‘‘Secretaria de medio ambiente y recursos naturals’’ (SEMARNAT) project SEMARNAT-2004-C01-257 and ‘‘Consejo Nacional de Ciencia y Tecnología’’ (CONACYT) project SEP-2004-C01-479991.I.N. -G. received a postdoctoral grant from Cinvestav while C.V. -E. and R.A. -H. received grant-aided support from CONACYT.
PY - 2008/3
Y1 - 2008/3
N2 - The soil of the former lake Texcoco is an extreme environment localized in the valley of Mexico City, Mexico. It is highly saline and alkaline, where Na+, Cl-, HCO 3 - and CO 3 2- are the predominant ions, with a pH ranging from 9.8 to 11.7 and electrolytic conductivities in saturation extracts from 22 to 150 dS m-1. Metagenomic DNA from the archaeal community was extracted directly from soil and used as template to amplify 16S ribosomal gene by PCR. PCR products were used to construct gene libraries. The ribosomal library showed that the archaeal diversity included Natronococcus sp., Natronolimnobius sp., Natronobacterium sp., Natrinema sp., Natronomonas sp., Halovivax sp., "Halalkalicoccus jeotgali" and novel clades within the family of Halobacteriaceae. Four clones could not be classified. It was found that the archaeal diversity in an alkaline-saline soil of the former lake Texcoco, Mexico, was low, but showed yet uncharacterized and unclassified species.
AB - The soil of the former lake Texcoco is an extreme environment localized in the valley of Mexico City, Mexico. It is highly saline and alkaline, where Na+, Cl-, HCO 3 - and CO 3 2- are the predominant ions, with a pH ranging from 9.8 to 11.7 and electrolytic conductivities in saturation extracts from 22 to 150 dS m-1. Metagenomic DNA from the archaeal community was extracted directly from soil and used as template to amplify 16S ribosomal gene by PCR. PCR products were used to construct gene libraries. The ribosomal library showed that the archaeal diversity included Natronococcus sp., Natronolimnobius sp., Natronobacterium sp., Natrinema sp., Natronomonas sp., Halovivax sp., "Halalkalicoccus jeotgali" and novel clades within the family of Halobacteriaceae. Four clones could not be classified. It was found that the archaeal diversity in an alkaline-saline soil of the former lake Texcoco, Mexico, was low, but showed yet uncharacterized and unclassified species.
KW - 16S rRNA
KW - Alkaline-saline soil
KW - Archaeal community
KW - Clone library
UR - http://www.scopus.com/inward/record.url?scp=40349083715&partnerID=8YFLogxK
U2 - 10.1007/s00792-007-0121-y
DO - 10.1007/s00792-007-0121-y
M3 - Artículo
C2 - 18097633
SN - 1431-0651
VL - 12
SP - 247
EP - 254
JO - Extremophiles
JF - Extremophiles
IS - 2
ER -