Photodynamic therapy in a cervico-uterine carcer cell line (HeLa) implanted in NU/NU mice

Pp IX accumulation reduced by ALA (I.P. and I.T.) was tested in a cell line of cervix carcinoma (HeLa) implanted in nu/nu mice, and irradiated by argon laser (wavelength 488 nm). It was found that the mice developed the tumor 17 days from its exposition to HeLa cells. The optimal concentration of ALA that induced maximum level of accumulation of PpIX in the tumors was 150 mg/kg by I.T. route. In ALA doses (150 and 175 mg/kg), it was observed a decrease in the tumoral area in the first three radiations at 234.36 J/cm². It was found that nu/nu swiss mice with implanted HeLa cells are a good model to study the epidermoide carcinoma (EC) because tumor development time is short although is valid only during 3 first months. The histology study shows that there is no cellular damage inmediates at the concentrations used. I.T. route does not allows a high PpIX accumulation in different organs, therefore this route is more safety. It was observed a decreasing size in the irradiated tumor by using an argon laser, this preliminaries results are indicating that would be possible to propose the use of this laser in PDT applied in EC.