TY - JOUR
T1 - Phospholipid membranes form specific nonbilayer molecular arrangements that are antigenic
AU - Aguilar, Leopolde
AU - Ortega-Pierres, Guadalupe
AU - Campos, Begoña
AU - Fonseca, Rocio
AU - Ibáñez, Miguel
AU - Wong, Carlos
AU - Farfán, Norberto
AU - Naciff, Jorge M.
AU - Kaetzel, Marcia A.
AU - Dedman, John R.
AU - Baeza, Isabel
PY - 1999/9/3
Y1 - 1999/9/3
N2 - Hexagonal phase (H(II))-preferring lipids such as phosphatidate, cardiolipin, and phosphatidylserine form nonbilayer molecular arrangements in lipid bilayers. While their presence in biological membranes has not been established, in vitro studies suggest that alterations in membrane properties modify their function. In this study, antiphospholipid monoclonal antibodies were developed against nonbilayer structures. One of the monoclonal antibodies identifies nonplanar surfaces in liposomes and in membranes of cultured cells. These results are the first evidence that natural membranes maintain a fragile balance between bilayer and nonbilayer lipid arrangements. Therefore, these antibodies can be used to evaluate the role of H(II)- preferring lipids in the modulation of membrane activities. Our studies demonstrated that nonplanar surfaces are highly immunogenic. Although these structures are normally transient, their formation can be stabilized by temperature variations, drugs, antibiotics, apolar peptides, and divalent cations. Our studies demonstrated that abnormal exposure of nonbilayer arrangements may induce autoimmune responses as found in the antiphospholipid syndrome.
AB - Hexagonal phase (H(II))-preferring lipids such as phosphatidate, cardiolipin, and phosphatidylserine form nonbilayer molecular arrangements in lipid bilayers. While their presence in biological membranes has not been established, in vitro studies suggest that alterations in membrane properties modify their function. In this study, antiphospholipid monoclonal antibodies were developed against nonbilayer structures. One of the monoclonal antibodies identifies nonplanar surfaces in liposomes and in membranes of cultured cells. These results are the first evidence that natural membranes maintain a fragile balance between bilayer and nonbilayer lipid arrangements. Therefore, these antibodies can be used to evaluate the role of H(II)- preferring lipids in the modulation of membrane activities. Our studies demonstrated that nonplanar surfaces are highly immunogenic. Although these structures are normally transient, their formation can be stabilized by temperature variations, drugs, antibiotics, apolar peptides, and divalent cations. Our studies demonstrated that abnormal exposure of nonbilayer arrangements may induce autoimmune responses as found in the antiphospholipid syndrome.
UR - http://www.scopus.com/inward/record.url?scp=0033520401&partnerID=8YFLogxK
U2 - 10.1074/jbc.274.36.25193
DO - 10.1074/jbc.274.36.25193
M3 - Artículo
SN - 0021-9258
VL - 274
SP - 25193
EP - 25196
JO - Journal of Biological Chemistry
JF - Journal of Biological Chemistry
IS - 36
ER -