TY - JOUR
T1 - Phenoloxidase activity in larval and juvenile homogenates and adult plasma and haemocytes of bivalve molluscs
AU - Luna-González, Antonio
AU - Maeda-Martínez, Alfonso N.
AU - Vargas-Albores, Francisco
AU - Ascencio-Valle, Felipe
AU - Robles-Mungaray, Miguel
N1 - Funding Information:
The authors are grateful to Cultivos del Mar Sudcaliforniano S. A. de C. V. and to Sol Azul S. A. de C. V. for providing broodstock and experimental organisms employed in this study. This work was supported by Consejo Nacional de Ciencia y Tecnologı́a, Mexico (CONACyT, project G-33953-B) and a PhD-student grant to A. Luna, and by CIBNOR project PAC-5. Thanks to Dr Jorge Hernández López for his critical review and comments on a previous version of this manuscript. CIBNOR editing staff improved the English language text.
PY - 2003/10
Y1 - 2003/10
N2 - Phenoloxidase (PO) activity was studied in larval and juvenile homogenates and in the plasma and haemocytes of adult Crassostrea gigas, Argopecten ventricosus, Nodipecten subnodosus, and Atrina maura. Samples were tested for the presence of PO activity by incubation with the substrate L-3, 4-dihydroxyphenylalanine using trypsin, α-chymotrypsin, laminarin, lipopolysaccharides (LPS), and sodium dodecyl sulphate (SDS) to elicit activation of prophenoloxidase (proPO) system. PO activity was not detected in larval homogenate. In juvenile homogenate, PO activity was found only in C. gigas and N. subnodosus. PO activity was present in adult samples and was enhanced by elicitors in the plasma of all species tested, but in haemocyte lysate supernatant (HLS) of only N. subnodosus. Activation of proPO by laminarin was suppressed by a protease inhibitor cocktail (P-2714) in plasma and HLS of all species tested.
AB - Phenoloxidase (PO) activity was studied in larval and juvenile homogenates and in the plasma and haemocytes of adult Crassostrea gigas, Argopecten ventricosus, Nodipecten subnodosus, and Atrina maura. Samples were tested for the presence of PO activity by incubation with the substrate L-3, 4-dihydroxyphenylalanine using trypsin, α-chymotrypsin, laminarin, lipopolysaccharides (LPS), and sodium dodecyl sulphate (SDS) to elicit activation of prophenoloxidase (proPO) system. PO activity was not detected in larval homogenate. In juvenile homogenate, PO activity was found only in C. gigas and N. subnodosus. PO activity was present in adult samples and was enhanced by elicitors in the plasma of all species tested, but in haemocyte lysate supernatant (HLS) of only N. subnodosus. Activation of proPO by laminarin was suppressed by a protease inhibitor cocktail (P-2714) in plasma and HLS of all species tested.
KW - Argopecten ventricosus
KW - Atrina maura
KW - Crassostrea gigas
KW - Haemocytes
KW - Immunology
KW - Juveniles
KW - Larvae
KW - Nodipecten subnodosus
KW - Prophenoloxidase
UR - http://www.scopus.com/inward/record.url?scp=0141483718&partnerID=8YFLogxK
U2 - 10.1016/S1050-4648(02)00165-1
DO - 10.1016/S1050-4648(02)00165-1
M3 - Artículo
C2 - 12969649
SN - 1050-4648
VL - 15
SP - 275
EP - 282
JO - Fish and Shellfish Immunology
JF - Fish and Shellfish Immunology
IS - 4
ER -