TY - JOUR
T1 - Oxidative stress, cytoxicity, and cell mortality induced by nano-sized lead in aqueous suspensions
AU - Cornejo-Garrido, Hilda
AU - Kibanova, Daria
AU - Nieto-Camacho, Antonio
AU - Guzmán, José
AU - Ramírez-Apan, Teresa
AU - Fernández-Lomelín, Pilar
AU - Garduño, Maria Laura
AU - Cervini-Silva, Javiera
N1 - Funding Information:
HC-G gratefully acknowledges the support of an undergraduate fellowship from DGAPA-UNAM. The authors are most grateful to Drs. Ben Gilbert and Zhao Hao (LBNL) for technical assistance. The authors would like to express their sincere appreciation to Dr. Rebecca Sutton (Environmental Working Group, Oakland, CA) who provided insightful comments and helpful suggestions which have substantially improved this manuscript. The authors thank Lic. María del Rocío Galindo Ortega (UAM-Cuajimalpa), and M. in Sc. Claudia Rivera Cerecedo and Héctor Malagon Rivero (Bioterio, Instituto de Fisiología Celular, UNAM) for technical assistance. This project was supported in part by Universidad Autónoma Metropolitana Unidad Cuajimalpa and ECACORE 2020 (SEMARNAT CONACYT 23496).
PY - 2011/9
Y1 - 2011/9
N2 - This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6h after incubation aqueous suspensions bearing nano-sized PbO2, soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1nmol TBARSmgprotein-1, respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO2 led to cell-growth inhibition values (%) ca ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however.
AB - This paper reports on the effect of aqueous and nano-particulated Pb on oxidative stress (lipid peroxidation), cytoxicity, and cell mortality. As determined by the Thiobarbituric Acid Reactive Substances (TBARS) method, only 6h after incubation aqueous suspensions bearing nano-sized PbO2, soluble Pb(II), and brain-homogenate only suspensions, were determined to contain as much as ca. 7, 5, and 1nmol TBARSmgprotein-1, respectively. Exposure of human cells (central nervous system, prostate, leukemia, colon, breast, lung cells) to nano-PbO2 led to cell-growth inhibition values (%) ca ≤18.7%. Finally, as estimated by the Artemia salina test, cell mortality values were found to show high-survival larvae rates. Microscopic observations revealed that Pb particles were swallowed, but caused no mortality, however.
KW - Biological activity
KW - Lead nanoparticles
KW - Lipid peroxidation
KW - Stable nanoparticles
KW - Water-stable
UR - http://www.scopus.com/inward/record.url?scp=79961207351&partnerID=8YFLogxK
U2 - 10.1016/j.chemosphere.2011.05.018
DO - 10.1016/j.chemosphere.2011.05.018
M3 - Artículo
C2 - 21640370
SN - 0045-6535
VL - 84
SP - 1329
EP - 1335
JO - Chemosphere
JF - Chemosphere
IS - 10
ER -