Osteoblast cell response on the Ti6Al4V alloy heat-treated

In an effort to examine the effect of the microstructural changes of the Ti6Al4V alloy, two heat treatments were carried out below (Ti6Al4V₈₀₀) and above (Ti6Al4V₁₀₅₀) its β-phase transformation temperature. After each treatment, globular and lamellar microstructures were obtained. Saos-2 pre-osteoblast human osteosarcoma cells were seeded onto Ti6Al4V alloy disks and immersed in cell culture for 7 days. Electrochemical assays in situ were performed using OCP and EIS measurements. Impedance data show a passive behavior for the three Ti6Al4V alloys; additionally, enhanced impedance values were recorded for Ti6Al4V₈₀₀ and Ti6Al4V₁₀₅₀ alloys. This passive behavior in culture medium is mostly due to the formation of TiO₂ during their sterilization. Biocompatibility and cell adhesion were characterized using the SEM technique; Ti6Al4V as received and Ti6Al4V₈₀₀ alloys exhibited polygonal and elongated morphology, whereas Ti6Al4V₁₀₅₀ alloy displayed a spherical morphology. Ti and O elements were identified by EDX analysis due to the TiO₂ and signals of C, N and O, related to the formation of organic compounds from extracellular matrix. These results suggest that cell adhesion is more likely to occur on TiO₂ formed in discrete α-phase regions (hcp) depending on its microstructure (grains).