Optimization of the ultrafiltration in fermentation broths

Tangential flow filtration operations were studied to concentrate fermentation broths Phaffia rhodozyma. The first part discusses the methodology used to determine the best conditions as transmembrane pressure, feed flow, temperature and pH to maximize the filtration flux. A comparison is made between the ultrafiltration and microfiltration based on the flux. In the second part, we discuss the scaling of the microfiltration and ultrafiltration using the mass transfer coefficient of polarizing gel model as a criterion for scaling. The final equation is deduced and also shows the sequence of calculation to determine the flow of feed which must operate pilot cartridges. Fluxes obtained at laboratory and pilot levels are compared and analyzes the importance of this criterion. The third part is focused on obtaining a model of resistance. With this model, it is possible to estimate the value of the flux at both laboratory and pilot levels under any combination of operating conditions. It explains the methodology used for the determination of the components of the model and empirical correlations are developed based on operating conditions. The final part deals with the optimization of energy consumption in tangential flow filtration operations. It explains the importance of energy consumption rate for the economy of the operation. Correlations are obtained for density, viscosity and rheological properties of fermentation broths in terms of cell concentration. These correlations are necessary to determine the pumping energy consumption per unit volume filtered. Operating conditions that minimize energy consumption are analyzed.