TY - JOUR
T1 - Optimization of biomass production by ustilago tnaydis in submerged culture using Taguchi experimental design
AU - Cornejo-Mazón, Maribel
AU - Jaramillo-Flores, Ma Eugenia
AU - Villa-Tanaca, Lourdes
AU - Hernández-Sánchez, Humberto
PY - 2008
Y1 - 2008
N2 - The aim of this study was to optimize the submerged culture conditions in baffled Erlenmeyer flasks for biomass production by U. maydis FBI 2 by L 8 Taguchi orthogonal array experimental design using YPD broth (yeast extract-peptone-dextrose) as a basal medium. The optimized conditions (glucose 40 g L-1, [Cu] 0.005 g L-1, pH 7, orbital shaking speed 200 rpm, temperature 32°C) predicted a final biomass concentration of 13.88 g L-1 after 48 h. When these conditions were tested experimentally, a real final biomass concentration of 15.67 g L-1 was obtained and the microorganism presented a specific growth rate μ = 0.03 h-1.
AB - The aim of this study was to optimize the submerged culture conditions in baffled Erlenmeyer flasks for biomass production by U. maydis FBI 2 by L 8 Taguchi orthogonal array experimental design using YPD broth (yeast extract-peptone-dextrose) as a basal medium. The optimized conditions (glucose 40 g L-1, [Cu] 0.005 g L-1, pH 7, orbital shaking speed 200 rpm, temperature 32°C) predicted a final biomass concentration of 13.88 g L-1 after 48 h. When these conditions were tested experimentally, a real final biomass concentration of 15.67 g L-1 was obtained and the microorganism presented a specific growth rate μ = 0.03 h-1.
KW - Biomass
KW - Optimization
KW - Submerged culture
KW - Taguchi
KW - Ustilago maydis
UR - http://www.scopus.com/inward/record.url?scp=67651030921&partnerID=8YFLogxK
U2 - 10.3923/biotech.2008.818.821
DO - 10.3923/biotech.2008.818.821
M3 - Artículo
SN - 1682-296X
VL - 7
SP - 818
EP - 821
JO - Biotechnology
JF - Biotechnology
IS - 4
ER -