TY - JOUR
T1 - Operational performance of the onchocerca volvulus "OEPA" Ov16 ELISA serological assay in mapping, guiding decisions to stop mass drug administration, and posttreatment surveillance surveys
AU - Richards, Frank O.
AU - Katabarwa, Moses
AU - Bekele, Firdaweke
AU - Tadesse, Zerihun
AU - Mohammed, Aderajew
AU - Sauerbrey, Mauricio
AU - Dominguez-Vazquez, Alfredo
AU - Rodriguez-Perez, Mario A.
AU - Fernández-Santos, Nadia A.
AU - Rizzo, Nidia
AU - Martínez, Harland R.Schuler
AU - Silva, Raquel Lovato
AU - Monroy, Zoraida Morales
AU - Habomugisha, Peace
AU - Oguttu, David W.
AU - Zarroug, Issam M.A.
AU - Aziz, Nabil A.
AU - Unnasch, Thomas R.
N1 - Publisher Copyright:
© Copyright 2018 by The American Society of Tropical Medicine and Hygiene.
PY - 2018
Y1 - 2018
N2 - Onchocerciasis is a neglected tropical disease targeted for elimination. The World Health Organization (WHO) has developed guidelines for the verification of onchocerciasis elimination that include entomological and epidemiological criteria. The latter require demonstrating with statistical confidence that the infection prevalence inchildrenisless than 0.1%, necessitating an assay with a high degree of specificity. We present an analysis of the performance of the Onchocerciasis Elimination Program for the Americas (OEPA) version of the Ov16 enzyme-linked immunosorbant assay (ELISA) when used under operational conditions. In Africa and Latin America, the assay demonstrated 99.98% specificity in 69, 888 children in 20 foci where transmission was believed to be interrupted. The assay produced a prevalence estimate equal to that of skin snip microscopy when applied in putatively hypo-endemic zones of Ethiopia. The OEPA Ov16 ELISA demonstrated the specificity required to be effectively deployed to verify transmission elimination under the WHO guidelines, while exhibiting a sensitivity equivalent to skin snip microscopy to identify hypo-endemic areas.
AB - Onchocerciasis is a neglected tropical disease targeted for elimination. The World Health Organization (WHO) has developed guidelines for the verification of onchocerciasis elimination that include entomological and epidemiological criteria. The latter require demonstrating with statistical confidence that the infection prevalence inchildrenisless than 0.1%, necessitating an assay with a high degree of specificity. We present an analysis of the performance of the Onchocerciasis Elimination Program for the Americas (OEPA) version of the Ov16 enzyme-linked immunosorbant assay (ELISA) when used under operational conditions. In Africa and Latin America, the assay demonstrated 99.98% specificity in 69, 888 children in 20 foci where transmission was believed to be interrupted. The assay produced a prevalence estimate equal to that of skin snip microscopy when applied in putatively hypo-endemic zones of Ethiopia. The OEPA Ov16 ELISA demonstrated the specificity required to be effectively deployed to verify transmission elimination under the WHO guidelines, while exhibiting a sensitivity equivalent to skin snip microscopy to identify hypo-endemic areas.
UR - http://www.scopus.com/inward/record.url?scp=85053027844&partnerID=8YFLogxK
U2 - 10.4269/ajtmh.18-0341
DO - 10.4269/ajtmh.18-0341
M3 - Artículo
C2 - 30014821
AN - SCOPUS:85053027844
SN - 0002-9637
VL - 99
SP - 749
EP - 752
JO - American Journal of Tropical Medicine and Hygiene
JF - American Journal of Tropical Medicine and Hygiene
IS - 3
ER -