Obtaining mutants for protoplast fusion of gibberellin-forming itgibberella fujikuroi strains

K. Bermudez Torres; B. Brückner; B. Meier

doi:10.1007/BF02950779

Obtaining mutants for protoplast fusion of gibberellin-forming itgibberella fujikuroi strains

K. Bermudez Torres, B. Brückner, B. Meier

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Resumen

Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.

Idioma original	Inglés
Páginas (desde-hasta)	83-95
Número de páginas	13
Publicación	Applied Biochemistry and Biotechnology
Volumen	33
N.º	2
DOI	https://doi.org/10.1007/BF02950779
Estado	Publicada - may. 1992
Publicado de forma externa	Sí

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title = "Obtaining mutants for protoplast fusion of gibberellin-forming itgibberella fujikuroi strains",

abstract = "Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.",

keywords = "G. fujikuroi, albino mutants, auxotrophic, drug resistant, gibberellic acid, nitrate-nonutilizing, protoplast fusion",

author = "{Bermudez Torres}, K. and B. Br{\"u}ckner and B. Meier",

year = "1992",

month = may,

doi = "10.1007/BF02950779",

language = "Ingl{\'e}s",

volume = "33",

pages = "83--95",

journal = "Applied Biochemistry and Biotechnology",

issn = "0273-2289",

number = "2",

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TY - JOUR

T1 - Obtaining mutants for protoplast fusion of gibberellin-forming itgibberella fujikuroi strains

AU - Bermudez Torres, K.

AU - Brückner, B.

AU - Meier, B.

PY - 1992/5

Y1 - 1992/5

N2 - Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.

AB - Auxotrophic, drug-resistant, nitrate-nonutilizing, and albino mutants have been isolated in Gibberella fujikuroi following UV mutagenesis. Protoplasts of complementing auxotrophic strains, mutants with resistance markers, or mutants blocked in different steps of the nitrate assimilatory pathway have been fused to form heterokaryons, diploids, or recombinant haploids. The properties of fusant strains, including gibberellic acid productivity, have been examined and compared to parent strains.

KW - G. fujikuroi

KW - albino mutants

KW - auxotrophic

KW - drug resistant

KW - gibberellic acid

KW - nitrate-nonutilizing

KW - protoplast fusion

UR - http://www.scopus.com/inward/record.url?scp=24444444485&partnerID=8YFLogxK

U2 - 10.1007/BF02950779

DO - 10.1007/BF02950779

M3 - Artículo

SN - 0273-2289

VL - 33

SP - 83

EP - 95

JO - Applied Biochemistry and Biotechnology

JF - Applied Biochemistry and Biotechnology

IS - 2

ER -

Obtaining mutants for protoplast fusion of gibberellin-forming itgibberella fujikuroi strains

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