TY - JOUR
T1 - Natural vertical transmission of zika virus in larval aedes aegypti populations, morelos, mexico
AU - Izquierdo-Suzán, Mónica
AU - Zárate, Selene
AU - Torres-Flores, Jesús
AU - Correa-Morales, Fabián
AU - González-Acosta, Cassandra
AU - Sevilla-Reyes, Edgar E.
AU - Lira, Rosalia
AU - Alcaraz-Estrada, Sofía L.
AU - Yocupicio-Monroy, Martha
N1 - Publisher Copyright:
© 2019, Centers for Disease Control and Prevention (CDC). All rights reserved.
PY - 2019/8
Y1 - 2019/8
N2 - We characterized natural vertical transmission of Zika virus in pools of Aedes aegypti larvae hatched from eggs collected in Jojutla, Morelos, Mexico. Of the 151 pools analyzed, 17 tested positive for Zika virus RNA; infectious Zika virus was successfully isolated from 1 of the larvae pools (31N) in C6/36 cells. Real-time quantitative PCR and indirect immunofluorescence assays confirmed the identity of the isolate, named Zika virus isolate 31N; plaque assays in Vero cells demonstrated the isolate’s infectivity in a mammalian cell line. We obtained the complete genome of Zika virus isolate 31N by next-generation sequencing and identified 3 single-nucleotide variants specific to Zika virus isolate 31N using the meta-CATS tool. These results demonstrate the occurrence of natural vertical transmission of Zika virus in wild Ae. aegypti mosquitoes and suggest that this transmission mode could aid in the spread and maintenance of Zika virus in nature.
AB - We characterized natural vertical transmission of Zika virus in pools of Aedes aegypti larvae hatched from eggs collected in Jojutla, Morelos, Mexico. Of the 151 pools analyzed, 17 tested positive for Zika virus RNA; infectious Zika virus was successfully isolated from 1 of the larvae pools (31N) in C6/36 cells. Real-time quantitative PCR and indirect immunofluorescence assays confirmed the identity of the isolate, named Zika virus isolate 31N; plaque assays in Vero cells demonstrated the isolate’s infectivity in a mammalian cell line. We obtained the complete genome of Zika virus isolate 31N by next-generation sequencing and identified 3 single-nucleotide variants specific to Zika virus isolate 31N using the meta-CATS tool. These results demonstrate the occurrence of natural vertical transmission of Zika virus in wild Ae. aegypti mosquitoes and suggest that this transmission mode could aid in the spread and maintenance of Zika virus in nature.
UR - http://www.scopus.com/inward/record.url?scp=85069909460&partnerID=8YFLogxK
U2 - 10.3201/eid2508.181533
DO - 10.3201/eid2508.181533
M3 - Artículo
C2 - 31310224
AN - SCOPUS:85069909460
SN - 1080-6040
VL - 25
SP - 1477
EP - 1484
JO - Emerging Infectious Diseases
JF - Emerging Infectious Diseases
IS - 8
ER -
