TY - JOUR
T1 - Nanobiomechanical behavior of Fe3O4@SiO2and Fe3O4@SiO2-NH2nanoparticles over HeLa cells interfaces
AU - Camacho-Fernández, Juan Carlos
AU - González-Quijano, Génesis Karendash
AU - Séverac, Childérick
AU - Dague, Etienne
AU - Gigoux, Véronique
AU - Santoyo-Salazar, Jaime
AU - Martinez-Rivas, Adrian
N1 - Publisher Copyright:
© 2021 IOP Publishing Ltd.
PY - 2021/9/17
Y1 - 2021/9/17
N2 - In this work, we studied the impact of magnetic nanoparticles (MNPs) interactions with HeLa cells when they are exposed to high frequency alternating magnetic field (AMF). Specifically, we measured the nanobiomechanical properties of cell interfaces by using atomic force microscopy (AFM). Magnetite (Fe3O4) MNPs were synthesized by coprecipitation and encapsulated with silica (SiO2): Fe3O4@SiO2 and functionalized with amino groups (-NH2): Fe3O4@SiO2-NH2, by sonochemical processing. HeLa cells were incubated with or without MNPs, and then exposed to AMF at 37 C. A biomechanical analysis was then performed through AFM, providing the Young's modulus and stiffness of the cells. The statistical analysis (p < 0.001) showed that AMF application or MNPs interaction modified the biomechanical behavior of the cell interfaces. Interestingly, the most significant difference was found for HeLa cells incubated with Fe3O4@SiO2-NH2 and exposed to AMF, showing that the local heat of these MNPs modified their elasticity and stiffness.
AB - In this work, we studied the impact of magnetic nanoparticles (MNPs) interactions with HeLa cells when they are exposed to high frequency alternating magnetic field (AMF). Specifically, we measured the nanobiomechanical properties of cell interfaces by using atomic force microscopy (AFM). Magnetite (Fe3O4) MNPs were synthesized by coprecipitation and encapsulated with silica (SiO2): Fe3O4@SiO2 and functionalized with amino groups (-NH2): Fe3O4@SiO2-NH2, by sonochemical processing. HeLa cells were incubated with or without MNPs, and then exposed to AMF at 37 C. A biomechanical analysis was then performed through AFM, providing the Young's modulus and stiffness of the cells. The statistical analysis (p < 0.001) showed that AMF application or MNPs interaction modified the biomechanical behavior of the cell interfaces. Interestingly, the most significant difference was found for HeLa cells incubated with Fe3O4@SiO2-NH2 and exposed to AMF, showing that the local heat of these MNPs modified their elasticity and stiffness.
KW - AFM nanoindentations
KW - HeLa cells
KW - alternating magnetic fields
KW - biomechanics
KW - magnetic nanocore-shells
KW - magnetic nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85109865088&partnerID=8YFLogxK
U2 - 10.1088/1361-6528/ac0a13
DO - 10.1088/1361-6528/ac0a13
M3 - Artículo
C2 - 34111853
AN - SCOPUS:85109865088
SN - 0957-4484
VL - 32
JO - Nanotechnology
JF - Nanotechnology
IS - 38
M1 - 385702
ER -