TY - JOUR
T1 - Mycobacterium tuberculosis manipulates pulmonary APCs subverting early immune responses
AU - Garcia-Romo, Gina S.
AU - Pedroza-Gonzalez, Alexander
AU - Lambrecht, Bart N.
AU - Aguilar-Leon, Diana
AU - Estrada-Garcia, Iris
AU - Hernandez-Pando, Rogelio
AU - Flores-Romo, Leopoldo
N1 - Funding Information:
We thank Victor Rosales-Garcia, Juana Calderon-Amador and Luis Donis-Maturano for technical assistance. We are grateful to Dr. Graham A.W. Rook for reviewing and correcting the manuscript. This work was supported by the National Council for Science and Technology, CONACyT (Grant 104667 to Flores-Romo L and Grant 84456 to R. Hernandez-Pando).
PY - 2013/3
Y1 - 2013/3
N2 - Alveolar macrophages (AM) and dendritic cells (DCs) are the main antigen presenting cells (APCs) in the respiratory tract. Whereas macrophages have been extensively studied in tuberculosis, . in situ interactions of DC with . Mycobacterium tuberculosis (Mtb) are poorly explored. We aimed to characterize lung APCs during pulmonary tuberculosis in Balb/C mice infected with . Mtb H37Rv.Mtb-infection via the airways induced a delayed and continuous accumulation of DCs and AM in the lungs. While lung DCs increased after day 3 post-infection, macrophages increased after 2-3. weeks. Although both populations accumulated in lungs during the infection, DCs decreased in the late stages. Infection induced differential expression of co-stimulatory molecules in these lung APCs, decreasing to basal levels in both APCs in the late stages. A remarkable segregation was found regarding bacillary burden. Many macrophages contained numerous bacilli, but DC contained scarce mycobacteria or none. Mtb-infection also induced delayed accumulation of DC in draining lymph nodes. This delayed recruitment was not associated with a lack of IL-12p40, which was detected from day 3 post-infection. Although AM and lung DCs behave differently during pulmonary tuberculosis, Mtb apparently manipulates both lung APCs subverting early protective responses resulting in disease progression.
AB - Alveolar macrophages (AM) and dendritic cells (DCs) are the main antigen presenting cells (APCs) in the respiratory tract. Whereas macrophages have been extensively studied in tuberculosis, . in situ interactions of DC with . Mycobacterium tuberculosis (Mtb) are poorly explored. We aimed to characterize lung APCs during pulmonary tuberculosis in Balb/C mice infected with . Mtb H37Rv.Mtb-infection via the airways induced a delayed and continuous accumulation of DCs and AM in the lungs. While lung DCs increased after day 3 post-infection, macrophages increased after 2-3. weeks. Although both populations accumulated in lungs during the infection, DCs decreased in the late stages. Infection induced differential expression of co-stimulatory molecules in these lung APCs, decreasing to basal levels in both APCs in the late stages. A remarkable segregation was found regarding bacillary burden. Many macrophages contained numerous bacilli, but DC contained scarce mycobacteria or none. Mtb-infection also induced delayed accumulation of DC in draining lymph nodes. This delayed recruitment was not associated with a lack of IL-12p40, which was detected from day 3 post-infection. Although AM and lung DCs behave differently during pulmonary tuberculosis, Mtb apparently manipulates both lung APCs subverting early protective responses resulting in disease progression.
KW - Alveolar macrophages
KW - Dendritic cells
KW - In vivo
KW - Tuberculosis
UR - http://www.scopus.com/inward/record.url?scp=84873181431&partnerID=8YFLogxK
U2 - 10.1016/j.imbio.2012.05.022
DO - 10.1016/j.imbio.2012.05.022
M3 - Artículo
SN - 0171-2985
VL - 218
SP - 393
EP - 401
JO - Immunobiology
JF - Immunobiology
IS - 3
ER -