TY - JOUR
T1 - Multichromosomal genome structure and confirmation of diazotrophy in novel plant-associated Burkholderia species
AU - Martínez-Aguilar, Lourdes
AU - Díaz, Rafael
AU - Peña-Cabriales, Juan José
AU - Estrada-de Los Santos, Paulina
AU - Dunn, Michael F.
AU - Caballero-Mellado, Jesús
PY - 2008/7
Y1 - 2008/7
N2 - Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. 15N2 isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.
AB - Pulsed-field gel electrophoresis and 16S rRNA hybridization experiments showed that multichromosome genome structures and very large genome sizes (6.46 to 8.73 Mb) are prevalent in novel plant-associated Burkholderia species. 15N2 isotope dilution assays revealed unambiguous diazotrophy in these novel species. nifH gene sequence analysis, often used to determine phylogenetic relatedness among diazotrophs, showed tight clusters of Burkholderia species, which were clearly distinct from those of other diazotrophs.
UR - http://www.scopus.com/inward/record.url?scp=47749100130&partnerID=8YFLogxK
U2 - 10.1128/AEM.00201-08
DO - 10.1128/AEM.00201-08
M3 - Artículo
SN - 0099-2240
VL - 74
SP - 4574
EP - 4579
JO - Applied and Environmental Microbiology
JF - Applied and Environmental Microbiology
IS - 14
ER -