TY - JOUR
T1 - Morphological and Physicochemical Characterization of Agglomerates of Titanium Dioxide Nanoparticles in Cell Culture Media
AU - Freyre-Fonseca, Verónica
AU - Téllez-Medina, Darió I.
AU - Medina-Reyes, Estefany I.
AU - Cornejo-Mazón, Maribel
AU - López-Villegas, Edgar O.
AU - Alamilla-Beltrán, Liliana
AU - Ocotlán-Flores, José
AU - Chirino, Yolanda I.
AU - Gutiérrez-López, Gustavo F.
N1 - Publisher Copyright:
© 2016 Verónica Freyre-Fonseca et al.
PY - 2016
Y1 - 2016
N2 - Titanium dioxide nanoparticles (TiO2 NP) are possible carcinogenic materials (2B-IARC) and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2 NP by evaluating their morphometry, stability, and zeta potential (ζ) in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS) resulted to be 10-50 times larger than those obtained by digital image analysis (DIA) given the charged zone around particles. Fractal dimension (FD) was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci) which was approximately unchanged with time. All dispersions had ζ values around-30 mV at physiological pH (7.4) and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis). Results help in understanding the complex NP geometry-size-stability relationships when performing in vivo and in vitro environmental-toxicity works and help in supporting decisions on the usage of TiO2 NP.
AB - Titanium dioxide nanoparticles (TiO2 NP) are possible carcinogenic materials (2B-IARC) and their toxicity depends on shape, size, and electrical charge of primary NP and on the system formed by NP media. The aim of this work was to characterize agglomerates of three TiO2 NP by evaluating their morphometry, stability, and zeta potential (ζ) in liquid media and their changes with time. Sizes of agglomerates by dynamic light scattering (DLS) resulted to be 10-50 times larger than those obtained by digital image analysis (DIA) given the charged zone around particles. Fractal dimension (FD) was highest for agglomerates of spheres and belts in F12K, and in E171 in FBS media. E171 and belts increased FD with time. At time zero, using water as dispersant FD was larger for agglomerates of spheres than for of E171. Belts suspended in water had the smallest values of circularity (Ci) which was approximately unchanged with time. All dispersions had ζ values around-30 mV at physiological pH (7.4) and dispersions of NP in water and FBS showed maximum stability (Turbiscan Lab analysis). Results help in understanding the complex NP geometry-size-stability relationships when performing in vivo and in vitro environmental-toxicity works and help in supporting decisions on the usage of TiO2 NP.
UR - http://www.scopus.com/inward/record.url?scp=84962670074&partnerID=8YFLogxK
U2 - 10.1155/2016/5937932
DO - 10.1155/2016/5937932
M3 - Artículo
SN - 1687-4110
VL - 2016
JO - Journal of Nanomaterials
JF - Journal of Nanomaterials
M1 - 5937932
ER -