TY - JOUR
T1 - Morphological and biochemical effects of weekend alcohol consumption in rats
T2 - Role of concentration and gender
AU - Morales-González, José A.
AU - Sernas-Morales, María de Lourdes
AU - Morales-González, Ángel
AU - González-López, Laura Ligía
AU - Madrigal-Santillán, Eduardo Osiris
AU - Vargas-Mendoza, Nancy
AU - Fregoso-Aguilar, Tomás Alejandro
AU - Anguiano-Robledo, Liliana
AU - Madrigal-Bujaidar, Eduardo
AU - Álvarez-González, Isela
AU - Chamorro-Cevallos, Germán
N1 - Publisher Copyright:
© The Author(s) 2018.
PY - 2018/2/1
Y1 - 2018/2/1
N2 - AIM: To examine the association between weekend alcohol consumption and the biochemical and histological alterations at two different concentrations of alcohol in both genders in rats. METHODS: Wistar rats weighing 170-200 g were divided into groups as follows: (1) Control groups; and (2) weekend alcohol-consumption group: 2 d/weekly per 12 wk, at two different concentrations: (1) Group of males or females with a consumption of a solution of alcohol at 40%; and (2) group of males or females with a consumption of a solution of alcohol at 5%. At the end of the experiment, serum and liver samples were obtained. The following enzymes and metabolites were determined in serum: Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Lactate Dehydrogenase, and Gamma-Glutamyltransferase, and glucose, triglycerides, cholesterol, bilirubin, and albumin. Liver samples from each group were employed to analyze morphological abnormalities by light microscopy. RESULTS: In all of the weekend alcohol-consumption groups, AST activity presented a significant, 10-fold rise. Regarding ALT activity, the groups with weekend alcohol consumption presented a significant increase that was six times greater. Bilirubin levels increased significantly in both groups of females. We observed a significant increase in the parameters of fatty change and inflammation due to weekend alcohol consumption. Only the group of females that consumed alcohol at 40% presented slight hepatocellular disorganization CONCLUSION: The results obtained herein provide solid evidence that weekend alcohol consumption gives rise to liver damage, demonstrated by biochemical and histological alterations, first manifested acutely, and prolonged weekend alcohol consumption can cause greater, irreversible damage.
AB - AIM: To examine the association between weekend alcohol consumption and the biochemical and histological alterations at two different concentrations of alcohol in both genders in rats. METHODS: Wistar rats weighing 170-200 g were divided into groups as follows: (1) Control groups; and (2) weekend alcohol-consumption group: 2 d/weekly per 12 wk, at two different concentrations: (1) Group of males or females with a consumption of a solution of alcohol at 40%; and (2) group of males or females with a consumption of a solution of alcohol at 5%. At the end of the experiment, serum and liver samples were obtained. The following enzymes and metabolites were determined in serum: Alanine Aminotransferase (ALT), Aspartate Aminotransferase (AST), Lactate Dehydrogenase, and Gamma-Glutamyltransferase, and glucose, triglycerides, cholesterol, bilirubin, and albumin. Liver samples from each group were employed to analyze morphological abnormalities by light microscopy. RESULTS: In all of the weekend alcohol-consumption groups, AST activity presented a significant, 10-fold rise. Regarding ALT activity, the groups with weekend alcohol consumption presented a significant increase that was six times greater. Bilirubin levels increased significantly in both groups of females. We observed a significant increase in the parameters of fatty change and inflammation due to weekend alcohol consumption. Only the group of females that consumed alcohol at 40% presented slight hepatocellular disorganization CONCLUSION: The results obtained herein provide solid evidence that weekend alcohol consumption gives rise to liver damage, demonstrated by biochemical and histological alterations, first manifested acutely, and prolonged weekend alcohol consumption can cause greater, irreversible damage.
KW - Damage
KW - Liver morphology
KW - Transaminases
KW - Weekend alcohol consumption
UR - http://www.scopus.com/inward/record.url?scp=85042793039&partnerID=8YFLogxK
U2 - 10.4254/wjh.v10.i2.297
DO - 10.4254/wjh.v10.i2.297
M3 - Artículo
C2 - 29527265
AN - SCOPUS:85042793039
SN - 1948-5182
VL - 10
SP - 297
EP - 307
JO - World Journal of Hepatology
JF - World Journal of Hepatology
IS - 2
ER -